Recognition of Human Cytomegalovirus by Human Primary Immunoglobulins Identifies an Innate Foundation to an Adaptive Immune Response
| Autor: | Ian N. Watt, Gary R. McLean, Ole Olsen, John W. Schrader, John S. Babcook, P. Rathanaswami, Kevin B. Leslie |
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| Rok vydání: | 2005 |
| Předmět: |
Human cytomegalovirus
DNA Complementary Molecular Sequence Data Immunology Immunoglobulin Variable Region Cytomegalovirus Immunoglobulins In Vitro Techniques Antibodies Viral Epitope Germline Viral Envelope Proteins medicine Humans Immunology and Allergy Amino Acid Sequence Codon Antigens Viral Gene Genetics Infectivity Base Sequence Sequence Homology Amino Acid biology Linear epitope Acquired immune system medicine.disease Adaptation Physiological Virology Immunity Innate Peptide Fragments Recombinant Proteins biology.protein Immunoglobulin Light Chains Antibody Immunoglobulin Heavy Chains |
| Zdroj: | The Journal of Immunology. 174:4768-4778 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Most primates, including humans, are chronically infected with cospecifically evolved, potentially pathogenic CMV. Abs that bind a 10-aa linear epitope (antigenic determinant 2 site 1) within the extracellular domain of human CMV glycoprotein B neutralize viral infectivity. In this study, we show that genes generated by recombinations involving two well-conserved human germline V elements (IGHV3-30 and IGKV3-11), and IGHJ4, encode primary Ig molecules that bind glycoprotein B at this key epitope. These particular VH, JH, and Vκ genes enable humans to generate through recombination and N nucleotide addition, a useful frequency of primary Igs that efficiently target this critical site on human CMV and thus confer an innate foundation for a specific adaptive response to this pathogen. |
| Databáze: | OpenAIRE |
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