| Autor: |
Albagli, Olivier, Maugein, Alicia, Huijbregts, Lukas, Bredel, Delphine, Carlier, Géraldine, Martin, Patrick, Scharfmann, Raphaël |
| Rok vydání: |
2019 |
| Předmět: |
|
| DOI: |
10.6084/m9.figshare.8286770.v1 |
| Popis: |
BXV1 efficiently mobilizes non-SIN, but not SIN, γ-retrovector in doubly transduced EndoC-βΗ2 cells. A. EndoC-βΗ2 cells transduced with the non-SIN γ-retrovector pPRIHy-TVA (EndoC-βΗ2-TVA) were further transduced with a SIN γ-retrovector (pSERS SF GFP-IRES-ZeoR). Each γ-retrovector harbors a distinct selectable marker (HygroR and ZeoR, respectively). Naive 293 T cells were next exposed to the conditioned medium (CM) of the doubly transduced EndoC-βΗ2 cells, then cultured in presence of either hygromycin or zeocin for 10 days, and finally fixed and colored with crystal violet. This experiment was done in duplicate, which gave similar results. B. The doubly transduced EndoC-βΗ2 cells display high GFP expression. This, together with their resistance to zeocin, confirms that they harbor pSERS SF GFP-IRES-ZeoR and express the corresponding transgenes. (DOCX 162 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
