Production of lysophosphatidic acid by lysophospholipase D in incubated plasma of spontaneously hypertensive rats and Wistar Kyoto rats
| Autor: | Kenji Fukuzawa, Hiroaki Fujimoto, Yuko Nishioka, Akira Tokumura, Osamu Yoshimoto, Maki Miyake |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
Aging medicine.medical_specialty Chromatography Gas Time Factors Phospholipid In Vitro Techniques Phospholipase Rats Inbred WKY General Biochemistry Genetics and Molecular Biology Pathogenesis chemistry.chemical_compound Spontaneously hypertensive rat Species Specificity Rats Inbred SHR Internal medicine Lysophosphatidic acid medicine Animals General Pharmacology Toxicology and Pharmaceutics Incubation Phosphoric Diester Hydrolases Fatty Acids General Medicine Rats Endocrinology Lysophosphatidylcholine Lysophospholipase chemistry Phosphatidylcholines lipids (amino acids peptides and proteins) Chromatography Thin Layer Lysophospholipids |
| Zdroj: | Life Sciences. 65:245-253 |
| ISSN: | 0024-3205 |
| Popis: | Lysophosphatidic acid has been identified as a vasopressor principle in incubated mammalian plasma and sera, and shown to be generated extracellulary by lysophospholipase D-like activity. In this study, we monitored the time course of changes in the major phospholipid fractions during incubation of plasma, and found that polyunsaturated lysophosphatidic acids accumulate more rapidly than saturated lysophosphatidic acids at expense of the corresponding lysophosphatidylcholines. We compared the phospholipase activities for producing bioactive LPA in age-matched spontaeneously hypertensive rats and Wistar Kyoto rats. The lysophospholipase D activity in rat plasma was found to be independent of strain and age. We suggest that lysophospholipase D functions in rat for persistent production of bioactive LPA in the circulation throughout life. However, our finding that production of LPA in spontaenously hypertensive rats was not greater than that in Wistar Kyoto rats does not seem to support the idea that increased production of LPA is involved in the pathogenesis of hypertension. |
| Databáze: | OpenAIRE |
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