Electron Transport and Oxidative Phosphorylation in the Blue-green Alga Anabaena variabilis
| Autor: | C. K. Leach, N. G. Carr |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Azides
Cyanide Antimycin A Oxidative phosphorylation Cyanobacteria Microbiology Oxidative Phosphorylation Electron Transport chemistry.chemical_compound Oxygen Consumption Menadione Oxidoreductase Rotenone chemistry.chemical_classification Chromatography Cyanides biology NADPH oxidation NAD biology.organism_classification Electron transport chain Succinate Dehydrogenase Biochemistry chemistry Oxidoreductases NADP Anabaena variabilis |
| Zdroj: | Journal of General Microbiology. 64:55-70 |
| ISSN: | 0022-1287 |
| DOI: | 10.1099/00221287-64-1-55 |
| Popis: | SUMMARY: The respiratory electron transport system involved in NADPH (2.0 nmoles/ min./mg.) and NADH (1.0 nmoles/min./mg.) oxidation in the dark that was operative in extracts of light-grown Anabaena variabilis has been examined. NADPH oxidation was inhibited 50% by cyanide (2x10-2m), rotenone (10-4 m); antimycin A, amytal and azide were markedly less inhibitory. NADPH: ferrocytochrome c oxidoreductase, NADPH: menadione oxido-reductase, ferrocytochrome c:oxygen oxidoreductase and succinic dehydrogenase were detected. A phosphorylation (0.4 nmoles/min./mg.), associated with NADPH oxidation, was measured, NADPH could be replaced by NADP and isocitrate. This phosphorylation was absolutely dependent upon oxygen and was inhibited 25% by carbonyl cyanide p-trifluoromethoxy-phenylhydrazone (5 μM). |
| Databáze: | OpenAIRE |
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