The t(14;18) defines a unique subset of diffuse large B-cell lymphoma with a germinal center B-cell gene expression profile
| Autor: | Ronald Levy, Diane L. Pickering, James O. Armitage, Izidore S. Lossos, Timothy C. Greiner, Louis M. Staudt, Dennis D. Weisenburger, Roger A. Warnke, Wing C. Chan, James C. Lynch, James Z. Huang, Ash A. Alizadeh, Warren G. Sanger |
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| Rok vydání: | 2002 |
| Předmět: |
Lymphoma
B-Cell Immunology Biology Polymerase Chain Reaction Biochemistry Translocation Genetic immune system diseases hemic and lymphatic diseases Gene expression medicine Humans B cell Chromosomes Human Pair 14 Gene Rearrangement Regulation of gene expression Large-cell lymphoma Chromosome Mapping Germinal center Cell Biology Hematology Gene rearrangement medicine.disease DNA Fingerprinting Molecular biology Genes bcl-2 Gene Expression Regulation Neoplastic Gene expression profiling medicine.anatomical_structure Diffuse large B-cell lymphoma Chromosomes Human Pair 8 |
| Zdroj: | Blood. 99:2285-2290 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v99.7.2285 |
| Popis: | Recently we have identified subgroups of de novo primary diffuse large B-cell lymphoma (DLBCL) based on complementary DNA microarray-generated gene expression profiles. To correlate the gene expression profiles with cytogenetic abnormalities in these DLBCLs, we examined the occurrence of the t(14;18)(q32;q21) in the 2 distinctive subgroups of DLBCL: one with the germinal center B-cell gene expression signature and the other with the activated B cell–like gene expression signature. The t(14;18) was detected in 7 of 35 cases (20%). All 7 t(14;18)-positive cases had a germinal center B-cell gene expression profile, representing 35% of the cases in this subgroup, and 6 of these 7 cases had very similar gene expression profiles. The expression of bcl-2 and bcl-6 proteins was not significantly different between the t(14;18)-positive and -negative cases, whereas CD10 was detected only in the group with the germinal center B-cell expression profile, and CD10 was most frequently expressed in the t(14;18)-positive cases. This study supports the validity of subdividing DLBCL into 2 major subgroups by gene expression profiling, with the t(14;18) being an important event in the pathogenesis of a subset of DLBCL arising from germinal center B cells. CD10 protein expression is useful in identifying cases of DLBCL with a germinal center B-cell gene expression profile and is often expressed in cases with the t(14;18). |
| Databáze: | OpenAIRE |
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