An experimental study and a randomized, double-blind, placebo-controlled clinical trial to evaluate the antisecretory activity of Lactobacillus acidophilus strain LB against nonrotavirus diarrhea
| Autor: | Luis E Sarrazin-Davila, Vanessa Liévin-Le Moal, Alain L. Servin |
|---|---|
| Přispěvatelé: | Codogno, Patrice, Signalisation et physiopathologie des cellules épithéliales, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Pediatrics, Hospital Clinica Kennedy |
| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.disease_cause 0302 clinical medicine Lactobacillus acidophilus [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Rotavirus Lactobacillus Medicine MESH: Double-Blind Method Cells Cultured MESH: Treatment Outcome 0303 health sciences education.field_of_study biology MESH: Escherichia coli MESH: Infant 3. Good health Diarrhea [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Treatment Outcome Rehydration Solutions [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases Female medicine.symptom MESH: Cells Cultured Population Context (language use) Placebo Microbiology 03 medical and health sciences Double-Blind Method 030225 pediatrics Escherichia coli Humans education [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology MESH: Humans 030306 microbiology business.industry Probiotics Infant biology.organism_classification MESH: Diarrhea Infantile MESH: Lactobacillus acidophilus MESH: Male Pediatrics Perinatology and Child Health Diarrhea Infantile business MESH: Female Bacteria MESH: Probiotics MESH: Rehydration Solutions |
| Zdroj: | Pediatrics Pediatrics, American Academy of Pediatrics, 2007, 120 (4), pp.e795-803. ⟨10.1542/peds.2006-2930⟩ |
| ISSN: | 1098-4275 0031-4005 |
| DOI: | 10.1542/peds.2006-2930⟩ |
| Popis: | OBJECTIVE. Previous studies have shown that selected strains of Lactobacillus have the capacity to antagonize rotavirus-induced diarrhea. However, only a few reports have documented their efficacy against nonrotavirus diarrhea. This study involved an experimental investigation and a clinical trial of the antisecretory activity of Lactobacillus acidophilus strain LB in the context of nonrotavirus diarrhea.METHODS. The activity of a culture of L acidophilus LB or of the lyophilized, heat-killed L acidophilus LB bacteria plus their spent culture medium was tested in inhibiting the formation of fluid-formed domes in cultured human intestinal Caco-2/TC7 cell monolayers infected with diarrheagenic, diffusely adhering Afa/Dr Escherichia coli C1845 bacteria. A randomized, double-blind, placebo-controlled clinical trial of male or female children who were 10 months of age and presented with nonrotavirus, well-established diarrhea was conducted to evaluate the therapeutic efficacy of a pharmaceutical preparation that contains 10 billion heat-killed L acidophilus LB plus 160 mg of spent culture medium.RESULTS. Infection of the cells with C1845 bacteria that were treated with L acidophilus LB culture or the lyophilized, heat-killed L acidophilus LB bacteria plus their culture medium produced a dosage-dependent decrease in the number of fluid-formed domes as compared with cells that were infected with untreated C1845 bacteria. The clinical results show that in selected and controlled homogeneous groups of children with well-established, nonrotavirus diarrhea, adding lyophilized, heat-killed L acidophilus LB bacteria plus their culture medium to a solution of oral rehydration solution shortened by 1 day the recovery time (ie, the time until the first normal stool was passed) as compared with children who received placebo oral rehydration solution.CONCLUSIONS. Heat-killed L acidophilus LB plus its culture medium antagonizes the C1845-induced increase in paracellular permeability in intestinal Caco-2/TC7 cells and produces a clinically significant benefit in the management of children with nonrotavirus, well-established diarrhea. |
| Databáze: | OpenAIRE |
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