Purification and Some Properties ofCandida albicansDNA Polymerases
| Autor: | Ferenc Hernádi, Tibor Schmidt, Erzsébet Jakab |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Aphidicolin
DNA polymerase DNA-Directed DNA Polymerase Biochemistry Sepharose chemistry.chemical_compound Candida albicans Genetics Sodium dodecyl sulfate Polyacrylamide gel electrophoresis Polymerase DNA Polymerase III chemistry.chemical_classification Molecular mass biology DNA Polymerase II Chromatography Ion Exchange DNA Polymerase I Molecular Weight Enzyme chemistry Chromatography Gel biology.protein Electrophoresis Polyacrylamide Gel |
| Zdroj: | Preparative Biochemistry. 21:105-123 |
| ISSN: | 0032-7484 |
| DOI: | 10.1080/10826069108018007 |
| Popis: | DNA polymerases of Candida albicans were purified to near homogeneity. Three well distinguished peaks of DNA polymerase activity (Enzyme I, II and III respectively) were obtained bv DEAE-Sephacel chromatography. This purification step was followed by column chromatographies on Sepharose 6B and denatured DNA-cellulose. The enzymes molecular mass and biochemical properties, including their inhibition by aphidicolin, were studied. Molecular mass was determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and was found to be 110 kDa for Enzyme I, 80 kDa for Enzyme II and 50 kDa for Enzyme III. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|