Multiple Polymorphic Loci for Molecular Typing of Strains of Mycobacterium leprae
| Autor: | Patrick J. Brennan, Varalakshmi D. Vissa, Hansuk Kim, Sang Nae Cho, Nathan A. Groathouse, Becky Rivoire, Hyeyoung Lee |
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| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Armadillos Minisatellite Repeat Minisatellite Repeats Biology Polymerase Chain Reaction law.invention Tandem repeat law Leprosy Genotype Animals Humans Mycobacterium leprae Polymerase chain reaction Genetics Polymorphism Genetic Molecular epidemiology Mycobacteriology and Aerobic Actinomycetes Sequence Analysis DNA biology.organism_classification Bacterial Typing Techniques Variable number tandem repeat Microsatellite Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Clinical Microbiology. 42:1666-1672 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.42.4.1666-1672.2004 |
| Popis: | The need for molecular tools for the differentiation of isolates of Mycobacterium leprae , the organism that causes leprosy, is urgent in view of the continuing high levels of new case detection, despite years of aggressive chemotherapy and the consequent reduction in the prevalence of leprosy. The slow onset of leprosy and the reliance on physical examination for detection of disease have restricted the epidemiological tracking necessary to understand and control transmission. Two genetic loci in several isolates of M. leprae have previously been demonstrated to contain variable-number tandem repeats (VNTRs). On the basis of these reports and the availability of the full genome sequence, multiple-locus VNTR analysis for strain typing has been undertaken. A panel of 11 short tandem repeat (STR) loci with repeat units of 1, 2, 3, 6, 12, 18, 21, and 27 bp from four clinical isolates of M. leprae propagated in armadillo hosts were screened by PCR. Fragment length polymorphisms were detected at 9 of the 11 loci by agarose gel electrophoresis. Sequencing of representative DNA products confirmed the presence of VNTRs between isolates. The application of nine new polymorphic STRs in conjunction with automated methods for electrophoresis and size determination allows greater discrimination between isolates of M. leprae and enhances the potential of this technique to track the transmission of leprosy. |
| Databáze: | OpenAIRE |
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