Effect of 1,25-dihydroxyvitamin D3 on mast cells tryptase in asthmatic guinea pigs
| Autor: | Rui-Qin Mai, Ling Shen, Gui-Dong Zhang, Xiao-He Zheng, Guo-Hong Zhang |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
medicine.medical_specialty
Mast cell tryptase Tryptase Expiratory airway resistance Group A Group B Guinea pig Airway resistance 1 25-Dihydroxyvitamin D3 Internal medicine medicine Asthmatic guinea pigs Medicine(all) biology medicine.diagnostic_test business.industry Stomach Alveolar septum General Medicine respiratory system Endocrinology medicine.anatomical_structure Bronchoalveolar lavage Immunology biology.protein business |
| Zdroj: | Asian Pacific Journal of Tropical Medicine. (6):494-497 |
| ISSN: | 1995-7645 |
| DOI: | 10.1016/j.apjtm.2015.05.006 |
| Popis: | Objective To explore the effect of 1,25-dihydroxyvitamin D3 on the mast cell tryptase (MCT) in asthmatic guinea pigs. Methods A total of 60 male or female healthy guinea pigs were randomly divided into control group (group A), asthmatic group (group B), and 1,25-dihydroxyvitamin D3 group (group C), with 20 cases in each group. To establish asthmatic guinea pig models, 1 mL peanut oil was filled into stomach in the morning in group A and group B, and 1 mL peanut oil with 1,25-dihydroxyvitamin D3 was filled into stomach in group C. Airway resistance (Re) of asthmatic guinea pigs was detected, and the bronchoalveolar lavage fluid (BALF) cells were counted. Lung tissue with HE and MCT immunohistochemical staining were used to observe the pathological changes in lung tissue and the distribution of MCT. Results After injection of different concentration of acetylcholine chloride, the Re in group B and group C were increased significantly compared with group A (P |
| Databáze: | OpenAIRE |
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