A polymerase chain reaction assay of cerebrospinal fluid in patients with suspected herpes simplex encephalitis
| Autor: | Neil E Anderson, K F Powell, M C Croxson |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Simplexvirus Pathology medicine.medical_specialty food.ingredient Adolescent viruses medicine.disease_cause Polymerase Chain Reaction Herpesviridae Virus law.invention food law Alphaherpesvirinae medicine Humans Polymerase chain reaction Aged Brain Diseases biology business.industry Infant Newborn Brain Herpes Simplex DNA Middle Aged biology.organism_classification medicine.disease Virology Psychiatry and Mental health Herpes simplex virus Blood-Brain Barrier Immunoglobulin G Encephalitis Female Surgery Neurology (clinical) business Meningitis Research Article |
| Zdroj: | Journal of Neurology, Neurosurgery & Psychiatry. 56:520-525 |
| ISSN: | 0022-3050 |
| DOI: | 10.1136/jnnp.56.5.520 |
| Popis: | A polymerase chain reaction (PCR) was used to detect herpes simplex virus (HSV) deoxyribonucleic acid (DNA) in CSF of 109 patients with possible herpes simplex encephalitis. HSV DNA was found in 20/109 patients. In 14 of these patients the diagnosis was confirmed by a rise in CSF antibodies, isolation of HSV from the brain, or both. In 3 patients CSF antibodies did not rise and 3 patients did not have a follow up lumbar puncture or a brain biopsy. In 19/20 patients HSV DNA was present in the first CSF specimen. The virus was identified as HSV I in 15 patients and HSV II in 4; the virus was not typed in the other patient. A possible diagnosis of herpes simplex encephalitis was not confirmed in the 89 PCR-negative patients. HSV DNA was present in CSF of 3 patients who had meningitis with herpetic genital infections but it was not found in 24 patients with other neurological diseases. The results suggest that the detection of HSV DNA in CSF using a PCR assay will be an accurate method of early diagnosis of herpes simplex encephalitis. |
| Databáze: | OpenAIRE |
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