Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals
| Autor: | F. R. Cardoso, Marcia Carneiro Valera, Lilian Eiko Maekawa, Cláudio Antonio Talge Carvalho, Sarah Almeida Coelho Oliveira, Stephanie F.P. Silva, Adriana Chung |
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| Rok vydání: | 2016 |
| Předmět: |
endotoxin
ginger Root canal chemistry.chemical_compound 0302 clinical medicine Candida albicans Enterococcus faecalis biomedical and dental materials Calcium hydroxide biology Chemistry chlorhexidine Chlorhexidine 04 agricultural and veterinary sciences bacterium Antimicrobial 040401 food science Corpus albicans Anti-Bacterial Agents antiinfective agent microbial sensitivity test medicine.anatomical_structure Root Canal Preparation medicine.drug Endodontic therapy in vitro study Microorganisms dental pulp cavity Microbial Sensitivity Tests Ginger In Vitro Techniques chemistry Microbiology Calcium Hydroxide root canal preparation 03 medical and health sciences 0404 agricultural biotechnology Zingiber officinale medicine Escherichia coli Humans human procedures General Dentistry Bacteria Root Canal Irrigants microbiology Intracanal medications 030206 dentistry biology.organism_classification Endotoxins drug effects Dental Pulp Cavity |
| Zdroj: | Scopus Repositório Institucional da UNITAU Universidade de Taubaté (UNITAU) instacron:UNITAU |
| ISSN: | 1526-3711 |
| Popis: | Aim The purpose of this in vitro study was to evaluate the antimicrobial activity of 2% chlorhexidine gel (CHX) as auxiliary chemical substance and intracanal medications on Candida albicans, Candida albicans, Enterococcus faecalis, Escherichia coli, and their endotoxins in the root canals. Materials and methods The study was conducted on 48 single-rooted human teeth divided into four groups (n = 12), according to intracanal medications used: (1) Calcium hydroxide + apyrogenic saline solution (Ca(OH)2 + SS), (2) 20% ginger glycolic extract (GEN), (3) calcium hydroxide + 20% ginger glycolic extract (Ca(OH)2 + GEN), (4) apyrogenic SS (control). Collections were made from the root canal content before preparation (baseline–S1), immediately after instrumentation (S2), 7 days after instrumentation (S3), after 14 days the action of intracanal medication (S4), and 7 days after removal of the intracanal medication (S5). The antimicrobial activity and endotoxin content were analyzed for all collections. The results were statistically analyzed by the Kruskal–Wallis and Dunn tests at a significance level of 5%. Results After instrumentation with CHX, there was complete elimination of E. coli and C. albicans, except for E. faecalis, which was significantly reduced and then completely eliminated after intracanal medication. There was significant reduction of endotoxin after instrumentation. Comparison of collection after instrumentation and intracanal medication revealed reduction of endotoxins in all groups; this reduction was greater in group Ca(OH)2 followed by the group GEN. Conclusion It was concluded that the instrumentation using CHX and intracanal medication used were able to eliminate the microorganisms from the root canal; the endotoxins were reduced, yet not completely eliminated. Clinical significance This study is important and relevant for searching alternatives during endodontic therapy, since it aims to study the effect of Zingiber officinale on microorganisms and endotoxins present in root canals. How to cite this article Valera MC, Oliveira SAC, Maekawa LE, Cardoso FGR, Chung A, Silva SFP, Carvalho CAT. Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals. J Contemp Dent Pract 2016; 17(2):114-118. |
| Databáze: | OpenAIRE |
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