Increased expression of antimüllerian hormone and its receptor in endometriosis
| Autor: | Paola Piomboni, Ana Luiza Lunardi Rocha, Felice Arcuri, Errico Zupi, Patrizia Carrarelli, Giuseppe Belmonte, Mauricio Simões Abrão, Felice Petraglia |
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| Rok vydání: | 2014 |
| Předmět: |
endometriosis
Adult Anti-Mullerian Hormone endocrine system medicine.medical_specialty Receptors Peptide ENDOMETRIOSE endocrine system diseases Endometriosis Endometrium Young Adult Transforming Growth Factor beta Internal medicine Receptors AMH Humans Medicine Prospective Studies AMHRII ovarian and deep endometriosis TGF-β superfamily Receptor Prospective cohort study medicine.diagnostic_test urogenital system business.industry Obstetrics and Gynecology Transforming growth factor beta superfamily medicine.disease female genital diseases and pregnancy complications medicine.anatomical_structure Endocrinology Biomarkers Female Receptors Transforming Growth Factor beta Gene Expression Regulation Reproductive Medicine Hysteroscopy Peptide Ovarian Endometriosis Immunohistochemistry Settore MED/40 - Ginecologia e Ostetricia business hormones hormone substitutes and hormone antagonists |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0015-0282 |
| Popis: | Objective To evaluate antimullerian hormone (AMH) and AMH receptor II (AMHRII) mRNA and protein expression in endometrium and in ovarian or deep lesions of women with endometriosis. Design Prospective study. Setting University hospitals in Italy and Brazil. Patients Patients with endometriosis (n = 55) and healthy women (n = 45). Interventions Specimens of endometrium obtained by hysteroscopy from patients with endometriosis and from healthy control subjects; specimens of ovarian endometriosis (n = 29) or of deep endometriosis (n = 26) were collected by laparoscopy. Serum samples were collected in some endometriotic patients (n = 23) and healthy control subjects (n = 20). Main Outcome Measure(s) AMH and AMHRII mRNA levels were evaluated by quantitative reverse-transcription polymerase chain reaction and protein localization by immunohistochemistry. AMH levels in tissue homogenates and in serum were assessed by ELISA. Result(s) Endometrium from women with endometriosis showed higher AMH and AMHRII mRNA levels than control women, with no significant differences between proliferative and secretory phases. Specimens collected from ovarian or deep endometriosis showed the highest AMH and AMHRII mRNA expression. Immunolocalization study confirmed the high AMH and AMHRII protein expression in endometriotic lesions. No difference of serum AMH levels between the groups was found. Conclusion(s) The increased AMH and AMHRII mRNA and protein expression in endometrium and in endometriotic lesions suggests a possible involvement of AMH in endometriosis. |
| Databáze: | OpenAIRE |
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