CO2-dependent carbon isotope fractionation in dinoflagellates relates to their inorganic carbon fluxes
| Autor: | Hoins, M., Eberlein, T., Van de Waal, D.B., Sluijs, A., Reichart, G.-J., Rost, B., NWO-DARWIN: Double Trouble: consequences of ocean acidification - Past, Present and Future: the Dinoflagellate Component, Marine palynology and palaeoceanography, Stratigraphy and paleontology |
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| Přispěvatelé: | NWO-DARWIN: Double Trouble: consequences of ocean acidification - Past, Present and Future: the Dinoflagellate Component, Marine palynology and palaeoceanography, Stratigraphy and paleontology, Aquatic Ecology (AqE) |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Bicarbonate Fractionation Aquatic Science 01 natural sciences chemistry.chemical_compound Total inorganic carbon Botany Organic matter CO2 uptake Ecology Evolution Behavior and Systematics chemistry.chemical_classification CCM biology 010604 marine biology & hydrobiology RuBisCO biology.organism_classification HCO3− uptake Alexandrium fundyense chemistry Isotopes of carbon international Environmental chemistry biology.protein Trochoidea (genus) Leakage 010606 plant biology & botany |
| Zdroj: | EPIC3Journal of Experimental Marine Biology and Ecology, ELSEVIER SCIENCE BV, 481, pp. 9-14, ISSN: 0022-0981 Journal of Experimental Marine Biology and Ecology, 481, 9. Elsevier ResearcherID Journal of Experimental Marine Biology and Ecology, 481, 9-14. Elsevier B.V. |
| ISSN: | 0022-0981 |
| DOI: | 10.1016/j.jembe.2016.04.001 |
| Popis: | Carbon isotope fractionation (εp) between the inorganic carbon source and organic matter has been proposed to be a function of pCO2. To understand the CO2-dependency of εp and species-specific differences therein, inorganic carbon fluxes in the four dinoflagellate species Alexandrium fundyense, Scrippsiella trochoidea, Gonyaulax spinifera and Protoceratium reticulatum have been measured by means of membrane-inlet mass spectrometry. In-vivo assays were carried out at different CO2 concentrations, representing a range of pCO2 from 180 to 1200μatm. The relative bicarbonate contribution (i.e. the ratio of bicarbonate uptake to total inorganic carbon uptake) and leakage (i.e. the ratio of CO2 efflux to total inorganic carbon uptake) varied from 0.2 to 0.5 and 0.4 to 0.7, respectively, and differed significantly between species. These ratios were fed into a single-compartment model, and εp values were calculated and compared to carbon isotope fractionation measured under the same conditions. For all investigated species, modeled and measured εp values were comparable (A. fundyense, S. trochoidea, P. reticulatum) and/or showed similar trends with pCO2 (A. fundyense, G. spinifera, P. reticulatum). Offsets are attributed to biases in inorganic flux measurements, an overestimated fractionation factor for the CO2-fixing enzyme RubisCO, or the fact that intracellular inorganic carbon fluxes were not taken into account in the model. This study demonstrates that CO2-dependency in εp can largely be explained by the inorganic carbon fluxes of the individual dinoflagellates. |
| Databáze: | OpenAIRE |
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