Alterations in the Expression of ELAM-1, ICAM-1 and VCAM-1 afterIn VitroInfection of Endothelial Cells with a Clinical Isolate of Human Cytomegalovirus
| Autor: | Shapour Shahgasempour, Helen M. Garnett, Salwa B. Woodroffe |
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| Rok vydání: | 1997 |
| Předmět: |
Human cytomegalovirus
Umbilical Veins T-Lymphocytes viruses Immunology Intercellular Adhesion Molecule-1 Cytomegalovirus Vascular Cell Adhesion Molecule-1 Biology Microbiology Monocytes Flow cytometry chemistry.chemical_compound Downregulation and upregulation Virology Cell Adhesion medicine Humans Endothelium VCAM-1 Cells Cultured ICAM-1 medicine.diagnostic_test Cell adhesion molecule virus diseases Flow Cytometry medicine.disease Molecular biology Up-Regulation Endothelial stem cell chemistry Culture Media Conditioned Cytomegalovirus Infections Leukocytes Mononuclear cardiovascular system E-Selectin |
| Zdroj: | Microbiology and Immunology. 41:121-129 |
| ISSN: | 0385-5600 |
| Popis: | Human cytomegalovirus (HCMV) infection of endothelial cells resulted in increased adhesion of the cells to peripheral blood leukocytes. It was demonstrated by flow cytometry that increased adhesiveness parallels the increased expression of cell surface adhesion molecules (ELAM-1, ICAM-1, VCAM-1). The increased adhesion of PMN and T-lymphocytes was due to upregulation in the expression of ELAM-1 and ICAM-1. The upregulation of VCAM-1 resulted in the increased adhesiveness of monocytes and T-lymphocytes to HCMV-infected HUVEC. The increased adhesiveness to leukocytes was caused by HCMV replication since endothelial cells exposed to HCMV-free supernatants and UV-inactivated HCMV did not show any increase in adhesiveness to any of the leukocytes tested. |
| Databáze: | OpenAIRE |
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