Performance Evaluation of a New Culture Colorimetric Detection Assay
| Autor: | Flavia Santi, Cartesio Favalli, Cartesio D'Agostini, Micol Piperno, Marco Favaro, Marco Ciotti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
lcsh:R5-920 biology 030306 microbiology business.industry General Medicine Mycoplasma hominis Mycoplasma biology.organism_classification medicine.disease_cause Virology 03 medical and health sciences Ureaplasma 0302 clinical medicine Ureaplasma parvum Medicine Gardnerella vaginalis Trichomonas vaginalis Original Article 030212 general & internal medicine business Mycoplasma genitalium lcsh:Medicine (General) human activities Ureaplasma urealyticum |
| Zdroj: | Eurasian Journal of Medicine, Vol 51, Iss 1, Pp 5-7 (2019) |
| ISSN: | 1308-8742 1308-8734 |
| Popis: | OBJECTIVE: To evaluate the performance of the culture colorimetric detection assay MYCO WELL D-ONE(®) (MWD-ONE), designed to detect sexually transmitted infections using real-time polymerase chain reaction (PCR) as a reference method. MATERIALS AND METHODS: One hundred and ten urogenital samples were screened for Gardnerella vaginalis (GV), Trichomonas vaginalis (TV), Mycoplasma hominis (MH), Mycoplasma spp., and Ureaplasma urealyticum (UU)/Ureaplasma parvum (UP) using the MWD-ONE and real-time PCR assays Gardnerella vaginalis/Lactobacillus species Real-TM Quant and Anyplex II STI-7 Detection, respectively. RESULTS: GV was detected in 33 samples by both the MWD-ONE and real-time PCR, while 6 samples gave discordant results. TV was detected by both MWD-ONE and Anyplex II STI-7 Detection kits in 3 samples, while 107 were negative. MH was detected by both methods in 5 cases, 4 samples gave discordant results, and 101 were negative. Mycoplasma genitalium (MG) was detected by Anyplex II STI-7 in 2 cases, 1 of which was detected as Mycoplasma spp. by MWD-ONE. Ten samples were positive by MWD-ONE, and 98 were negative with both assays. With regard to UU/UP, 24 cases were detected by MWD-ONE and Anyplex PCR, 25 by PCR only, 4 by MWD-ONE, and 57 tested negative with both methods. The positive predictive values (PPV) and negative predictive values (NPV) of the MWD-ONE assay for the pathogens tested were as following: GV, PPV 94.3%, NPV 94.7%; TV, PPV and NPV 100%; MH, PPV 71.4%, NPV 98.1%; Mycoplasma spp., PPV 9.1%, NPV 98.9%; and Ureaplasma spp., PPV 85.7 %, NPV 69.5 %. The agreement between the MWD-ONE and PCR was strong for GV and MH (k=0.8 and 0.7, respectively); perfect for TV (k=1); and moderate for UU/UP (k=0.4). CONCLUSION: MWD-ONE assay appears to be suitable for routine testing of sexually transmitted infections. |
| Databáze: | OpenAIRE |
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