Synergistic effects of green tea extract and paclitaxel in the induction of mitochondrial apoptosis in ovarian cancer cell lines
| Autor: | Vahideh Behmard, Ali Bakhshi, Zahra Zare, Omid Abazari, Saeede Yavari, Mojtaba Abbasi, Monireh Malekpour, Mohammad Panji, Tina Nayerpour dizaj, Maryam Shabanzadeh, Parisa Khanicheragh, Azadeh Safaeian, Hasan Nejadbiglari |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Paclitaxel Cell Survival Apoptosis Green tea extract Biology Pharmacology complex mixtures 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor Genetics medicine Humans Viability assay Cytotoxicity bcl-2-Associated X Protein Ovarian Neoplasms Tea Caspase 3 Plant Extracts Cytochromes c Polyphenols Drug Synergism General Medicine medicine.disease Antineoplastic Agents Phytogenic Caspase 9 Mitochondria 030104 developmental biology Proto-Oncogene Proteins c-bcl-2 chemistry Cell culture 030220 oncology & carcinogenesis Cancer cell Female Drug Screening Assays Antitumor Ovarian cancer Proto-Oncogene Proteins c-akt |
| Zdroj: | Gene. 787:145638 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2021.145638 |
| Popis: | Background Green tea is a natural compound with anti-neoplastic properties. Paclitaxel (PTX) is a natural anti-tumor medication used to manage patients with advanced ovarian cancer. This manuscript evaluated the cytotoxic effects of green tea extract combined with PTX drug in two human ovarian cancer cell lines (p53-negative cell line, SKOV-3; and mutant type p53 cell line, OVCAR-3) and underlying mechanisms. Methods The human ovarian cancer cell lines were treated with green tea extract, PTX, and green tea plus PTX for 24 h, and cell viability was assessed using the MTT method. Flow cytometric analyses were carried out to detect apoptosis. For the apoptotic process, quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting analysis were applied to study pAkt, Bax, Bcl-2, Cytochrome C (Cyt-C), cleaved-caspase-3, and cleaved-caspase-9 levels after drug treatments. Results Our results pointed out that various green tea (25 and 50 µg/ml) concentrations combined with PTX (20 and 40 µg/ml) synergistically inhibited cell viability of cancer cells more than green tea or PTX alone after 24 h of treatment. Also, green tea and PTX combination induced apoptosis in ovarian cancer cells by blocking the phosphorylation of Akt and the expression of Bcl-2 while inducing Bax, Cyt-C, cleaved-caspase 3, and cleaved-caspase 9. Conclusion Our results showed that the combination of green tea and PTX could be more potent than the individual drug to induce cytotoxicity and apoptosis in ovarian cancer cells. |
| Databáze: | OpenAIRE |
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