Simple potency test for LH-RH preparations
| Autor: | M.L. Givner, U.K. Banik |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Ovulation
Fluphenazine medicine.medical_specialty Chemistry media_common.quotation_subject Statistics as Topic Pharmacology Body weight General Biochemistry Genetics and Molecular Biology Rats Gonadotropin-Releasing Hormone Endocrinology Induced ovulation Internal medicine medicine Gonadorelin hydrochloride Animals Potency Biological Assay Female Gonadorelin acetate media_common medicine.drug |
| Zdroj: | Journal of Biological Standardization. 8:151-156 |
| ISSN: | 0092-1157 |
| DOI: | 10.1016/s0092-1157(80)80021-7 |
| Popis: | Proestrous rats, pretreated with fluphenazine dihydrochloride (FD) to block ovulation, were used to compare the ovulation-inducing activity of two synthetic LH-RH preparations; one was received from the National Institute for Biological Standards and Control labelled Gonadorelin acetate (77/596) and the other one was prepared in Ayerst Research Laboratories, Montreal, Canada, and labelled Gonadorelin hydrochloride (AY-24,031-A-23). It was found that FD blocked ovulation and graded doses of both LH-RH preparations induced dose-dependent ovulation in FD-treated rats. There were no significant differences between the biological potencies of both preparations. Both preparations, when administered intramuscularly at 1 μg/kg body weight, induced ovulation in 80% of the FD-pretreated proestrous rats. This simple test could be used to evaluate the biological potency of LH-RH and its analogues in less than 24 h. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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