M genotyping and DNA fingerprinting of Streptococcus pyogenes isolates from an area of central Italy
| Autor: | Maria Antonietta Mencarelli, R. Corbisiero, R. Signori, Carla Cellesi, A. Gistri, B. Marzocchi, A. Rossolini |
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| Rok vydání: | 1998 |
| Předmět: |
DNA
Bacterial Genotype Molecular epidemiology Streptococcus pyogenes Epidemiology DNA Patterns Biology medicine.disease_cause DNA Fingerprinting Microbiology Serology Infectious Diseases Italy DNA profiling Streptococcal Infections medicine Humans Typing Serotyping Genotyping Polymorphism Restriction Fragment Length Research Article |
| Popis: | M protein gene typing was used to analyse Streptococcus pyogenes clinical isolates collected between 1983 and 1995 in an area of central Italy from patients presenting different types of infections; the same isolates were also characterized by means of DNA fingerprinting.M type 1 was the most common (50% of study strains), followed by M types 4, 12 and 6. The proportion of M type 12 decreased with time, whereas M type 1 increased, in agreement with data obtained in many different areas. Most invasive strains belonged to types M1 (30%) and M12 (30%); on the other hand, the M1 type did frequently occur also among non-invasive isolates. DNA fingerprinting showed a correlation between M types and DNA patterns. This report provides epidemiological information from a geographic area not sampled recently, and further shows the usefulness of the M genotyping technique, which offers potential advantages over conventional serological typing methods. |
| Databáze: | OpenAIRE |
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