Microtubule disruption induced by estradiol in estrogen receptor-positive and -negative human breast cancer cell lines
| Autor: | Eriko Aizu-Yokota, Ken Ichinoseki, Yoshihiro Sato |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cytoplasm
Cancer Research medicine.medical_specialty Neoplasms Hormone-Dependent medicine.drug_class Estrogen receptor Breast Neoplasms Biology Microtubules Microtubule Internal medicine Tumor Cells Cultured medicine Humans skin and connective tissue diseases Diethylstilbestrol Interphase Cytoplasmic microtubule Centrosome Estradiol Cell growth Cell Membrane General Medicine Estradiol binding Cell biology Endocrinology Receptors Estrogen Estrogen Cell culture hormones hormone substitutes and hormone antagonists Intracellular |
| Zdroj: | Carcinogenesis. 15:1875-1879 |
| ISSN: | 1460-2180 0143-3334 |
| DOI: | 10.1093/carcin/15.9.1875 |
| Popis: | Effects of estrogens on the cytoplasmic microtubule network were examined by the indirect immunofluorescence method using anti-beta-tubulin antibody. Estradiol, a naturally occurring estrogen, decreased the amount of cytoplasmic microtubule fibers during interphase in the human breast cancer cell lines MCF-7 and MDA-MB-231. Since MDA-MB-231 is an estrogen receptor-negative cell line, estradiol-induced microtubule disruption seems to be independent of estradiol binding to receptors. The effective concentration of estradiol required for induction of microtubule disruption in 50% of the cells (EC50) was 81 microM for MCF-7 cells and 82 microM for MDA-MB-231 cells. A synthetic estrogen, diethylstilbestrol, also induced a decrease in microtubule fibers, with an EC50 value of 48 microM for MCF-7 cells and 50 microM for MDA-MB-231 cells. When estrogen-treated and microtubule-depolymerized cells were washed and the medium was replaced with fresh, intracellular microtubule networks reappeared within 3 h. When MCF-7 cells were cultured for 4 days with estradiol (50 microM), cell growth was completely inhibited. However, estrone affected the microtubule network and cell proliferation only slightly. These results suggest that estradiol-induced microtubule disruption is closely related to its inhibitory effect on cell growth. |
| Databáze: | OpenAIRE |
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