Impact of ischemia on sample quality of human pancreatic tissues
| Autor: | Yupei Zhao, Taiping Zhang, Chen Hao, Menghua Dai, Liu Lulu, Junchao Guo, Huang Dan, Dingyan Cao, Wenze Wang, Wenming Wu, Zhixuan Xuan, Jialin Jiang, Quan Liao, Peng Junya, Li Dongjing, Dan Guo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Pancreatic ductal adenocarcinoma Endocrinology Diabetes and Metabolism Ischemia Disease Specimen Handling 03 medical and health sciences Preclinical research 0302 clinical medicine medicine Humans Pancreas Aged Sample handling Aged 80 and over Hepatology business.industry Dissection Gastroenterology DNA Middle Aged medicine.disease Sample quality Gene Expression Regulation Neoplastic Pancreatic Neoplasms 030220 oncology & carcinogenesis Clinical diagnosis RNA 030211 gastroenterology & hepatology Female business Carcinoma Pancreatic Ductal Signal Transduction |
| Zdroj: | Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]. 20(2) |
| ISSN: | 1424-3911 |
| Popis: | Background Successful clinical evaluation of human tumors relies on proper handling of tissue samples to maximally preserve the cellular and metabolic states in vivo. Pancreatic samples are particularly sensitive to sample mishandling due to the abundance of digestive enzymes. We study how the duration of ischemia, in vivo and ex vivo, both of which are unavoidable lagging periods following surgical dissection, significantly impact the utility of pancreatic samples. Methods We systematically characterize a wide range of tissue integrity features, including histological patterns, cellular structures, DNA/RNA quality and activity of major signaling pathways in normal pancreases and pancreatic ductal adenocarcinoma (PDAC) tumor tissues from 41 patients with different ischemia. Results We reveal that tissues experiencing longer periods of ischemia exhibit significant deterioration and could potentially mislead disease diagnosis and preclinical research. Based on these analyses, we propose an optimal procedure that balances better clinical practice and high tissue sample quality. Conclusions Our work provides a guideline for pancreatic sample handling and could have wide implications in clinical diagnosis and translational research. |
| Databáze: | OpenAIRE |
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