Identification of aflatoxigenic fungi using polymerase chain reaction-based assay
| Autor: | M Marija Skrinjar, M Slavica Veskovic-Moracanin, Т Nevena Blagojev, M Vladislava Soso |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Aflatoxin
polymerase chain reaction Aspergillus flavus Fungus law.invention Microbiology 03 medical and health sciences chemistry.chemical_compound law lcsh:Technology (General) Gene Polymerase chain reaction 030304 developmental biology Regulator gene 2. Zero hunger 0303 health sciences biology 030306 microbiology General Engineering food and beverages aflatoxigenic fungi biology.organism_classification Aspergillus parasiticus chemistry lcsh:T1-995 DNA |
| Zdroj: | Acta Periodica Technologica, Vol 2014, Iss 45, Pp 259-269 (2014) Acta periodica technologica (2014) (45):259-269 |
| ISSN: | 1450-7188 |
| Popis: | As the aflatoxins represent a health-risk for humans because of their proven carcinogenicity, food-borne fungi that produce them as secondary metabolites, mainly Aspergillus flavus and Aspergillus parasiticus, have to be isolated and identified. The best argument for identifying problem fungi is that it indicates control points within the food system as part of a hazard analysis critical control point (HACCP) approach. This assumes there is a close link between fungus and toxin. Conventional methods for isolation and identification of fungi are time consuming and require admirably dedicated taxonomists. Hence, it is imperative to develop methodologies that are relatively rapid, highly specific and as an alternative to the existing methods. The polymerase chain reaction (PCR) facilitates the in vitro amplification of the target sequence. The main advantages of PCR is that organisms need not be cultured, at least not for a long time, prior to their detection, target DNA can be detected even in a complex mixture, no radioactive probes are required, it is rapid, sensitive and highly versatile. The gene afl-2 has been isolated and shown to regulate aflatoxin biosynthesis in A. flavus. Also, the PCR reaction was targeted against aflatoxin synthesis regulatory gene (aflR1) since these genes are nearly identical in A. flavus and A. parasiticus in order to indicate the possibility of detection of both the species with the same PCR system (primers/reaction). [Projekat Ministarstva nauke Republike Srbije, br. III46009] This article has been retracted. Link to the retraction 10.2298/APT1647265E |
| Databáze: | OpenAIRE |
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