Nitric oxide charged catheters as a potential strategy for prevention of hospital acquired infections

Autor: Mark Mizrahi, Jack Baniel, David Greenberg, Mary Ko, Daniel Kedar, Rachel Ozalvo, Gili Regev-Shoshani, Maya Moshe, Liat Shavit-Grievink, Ofer Yossepowitch, Andrei Nadu, David A. Lifshitz, Yossef Av-Gay, David Margel
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
medicine.medical_treatment
lcsh:Medicine
Yeast and Fungal Models
Pathology and Laboratory Medicine
medicine.disease_cause
Biochemistry
Bacterial Adhesion
Hemoglobins
Candida albicans
Medicine and Health Sciences
Enterococcus faecalis
Medicine
Prospective Studies
Reproductive System Procedures
lcsh:Science
Candida
Fungal Pathogens
Multidisciplinary
biology
Pseudomonas Aeruginosa
Neurochemistry
Middle Aged
Radical Prostatectomy
Corpus albicans
Bacterial Pathogens
Catheter
Experimental Organism Systems
Medical Microbiology
Anesthesia
Pathogens
Neurochemicals
Research Article
Biotechnology
Catheters
Urinary system
030106 microbiology
Surgical and Invasive Medical Procedures
Mycology
Urinary Catheters
Research and Analysis Methods
Nitric Oxide
Microbiology
Catheterization
03 medical and health sciences
Pseudomonas
Escherichia coli
Humans
Microbial Pathogens
Dialysis
Radical Surgery
Aged
Hematuria
Prostatectomy
Surgical Excision
Bacteria
business.industry
Pseudomonas aeruginosa
lcsh:R
Organisms
Fungi
Biofilm
Biology and Life Sciences
biology.organism_classification
Yeast
030104 developmental biology
Case-Control Studies
Catheter-Related Infections
Medical Devices and Equipment
lcsh:Q
business
Enterococcus
Neuroscience
Follow-Up Studies
Zdroj: PLoS ONE, Vol 12, Iss 4, p e0174443 (2017)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0174443
Popis: Background Catheter-Associated Hospital-Acquired Infections (HAI's) are caused by biofilm-forming bacteria. Using a novel approach, we generated anti-infective barrier on catheters by charging them with Nitric Oxide (NO), a naturally-produced gas molecule. NO is slowly released from the catheter upon contact with physiological fluids, and prevents bacterial colonization and biofilm formation onto catheter surfaces. Aims and methods The aim of the study was to assess the anti-infective properties of NO-charged catheters exposed to low concentration (up to 103 CFU/ml) of microbial cells in-vitro. We assessed NO-charged tracheal tubes using Pseudomonas aeruginosa, dialysis and biliary catheters using Escherichia coli, and urinary catheters using E. coli, Candida albicans or Enterococcus faecalis. Safety and tolerability of NO-charged urinary catheters were evaluated in a phase 1 clinical study in 12 patients. Six patients were catheterized with NO-charged catheters (NO-group), followed by 6 patients catheterized with regular control catheters (CT-group). Comparison of safety parameters between the study groups was performed. Results NO-charged tracheal, dialysis biliary and urinary catheters prevented P. aeruginosa, E. coli and C. albicans attachment and colonization onto their surfaces and eradicated corresponding planktonic microbial cells in the surrounding media after 24-48 hours, while E. faecalis colonization onto urinary catheters was reduced by 1 log compared to controls. All patients catheterized with an NO-charged urinary catheter successfully completed the study without experiencing NO-related AE's or serious AE's (SAE's). Conclusion These data highlight the potential of NO-based technology as potential platform for preventing catheter-associated HAI's.
Databáze: OpenAIRE
Externí odkaz: