Prospective lncRNA-miRNA-mRNA regulatory network of long non-coding RNA LINC00968 in non-small cell lung cancer A549 cells: A miRNA microarray and bioinformatics investigation
Autor: | Gang Chen, Wenjie Chen, Yong-Kun Wang, Jun Shang, Yu Zhang, Dong-Yao Li, Lei Luo, Shikang Li |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Biology Bioinformatics 03 medical and health sciences 0302 clinical medicine Carcinoma Non-Small-Cell Lung microRNA Genetics Humans Gene Regulatory Networks RNA Messenger KEGG Gene Regulation of gene expression Microarray analysis techniques Organelle lumen Computational Biology General Medicine Transfection Microarray Analysis Long non-coding RNA Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology A549 Cells 030220 oncology & carcinogenesis RNA Long Noncoding |
Zdroj: | International Journal of Molecular Medicine. |
ISSN: | 1791-244X 1107-3756 |
Popis: | Accumulating evidence suggests that the dysregulation of long non-coding RNAs (lncRNAs) serves vital roles in the incidence and progression of lung cancer. However, the molecular mechanisms of LINC00968, a recently identified lncRNA, remain unknown. The objective of present study was to investigate the role of a prospective lncRNA-miRNA‑mRNA network regulated by LINC00968 in non-small cell lung cancer cells. Following the transfection of lentiviruses carrying LINC00968 into A549 cells, the microRNA (miRNA) expression profile of the cells in response to the overexpression of LINC00968 was detected using an miRNA microarray. Five differentially expressed miRNAs (DEMs) with LINC00968 overexpression were obtained, including miR-9-3p, miR‑22-5p, miR-668-3p, miR‑3675-3p and miR-4536-3p. Five target prediction algorithms and three target validation algorithms were used to obtain 1,888 prospective target genes of the five DEMs. The result of Gene Ontology analysis suggested that these five DEMs were involved in complex cellular pathways, which included intracellular transport, organelle lumen and nucleotide binding. Furthermore, analysis of Kyoto Encyclopedia of Genes and Genomes pathways indicated that the five DEMs were important regulators in the adherens junction and focal adhesion. An lncRNA-miRNA-mRNA regulatory network and a protein-protein interaction network were then constructed. Eventually, a prospective lncRNA‑miRNA-mRNA regulatory network of LINC00968, three miRNAs (miR-9, miR-22 and miR-4536) and two genes (polo-like kinase 1 and exportin-1) was obtained following validation in the Cancer Genome Atlas database. These results may provide novel insights to support future research into lncRNA in lung cancer. |
Databáze: | OpenAIRE |
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