Molecular detection of Mycoplasma suis in captive white-lipped peccaries (Tayassu pecari) and wild boars (Sus scrofa) in Brazil
| Autor: | Rosangela Zacarias Machado, Guilherme Bim Dias, Luís Guilherme de Oliveira, Estevam Guilherme Lux Hoppe, Marcos Rogério André, Renan Bressianini do Amaral, Ivan Moura Lapera, Igor Renan Honorato Gatto |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial Swine Sus scrofa Immunology Population Zoology Captivity Bacteremia Real-Time Polymerase Chain Reaction Wild boar Microbiology Mycoplasma Mycoplasma suis RNA Ribosomal 16S biology.animal Animals Immunology and Allergy Mycoplasma Infections White-lipped peccary education Pathogen education.field_of_study Invasive species General Veterinary biology General Medicine Ribosomal RNA biology.organism_classification 16S ribosomal RNA Molecular Typing qPCR Infectious Diseases Tayassu pecari Herd Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) Brazil |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0147-9571 |
| Popis: | Made available in DSpace on 2019-10-06T16:14:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-04-01 Mycoplasma suis, the etiological agent of swine hemoplasmosis, is an epicellular bacterium that adheres to the surface of pig erythrocytes leading to deformations of the target cells. Little is known about the occurrence of M. suis in wild swine populations around the world, its economic impact on swine herds, and the risk of human infection. The aim of this study was to investigate, by quantitative real-time PCR (qPCR) based on the 16S rRNA gene, the occurrence of M. suis in a captive population of white-lipped peccaries (100 Tayassu pecari) and in free-living wild boars (14 Sus scrofa) in Brazil. None of the white-lipped peccaries were positive for M. suis, whereas seven (50%) wild boars were positive in qPCR assays. The quantification of M. suis-16S rRNA copies/μL ranged from 1.42 × 10° to 3.906 × 10 1 in positive animals, indicating a low bacteremia and a chronic carrier status in free-living wild boars. In conclusion, M. suis might be a non-frequent pathogen in wild suids maintained in captivity. Despite the low bacteremia, the prevalence of M. suis in wild boar population in Brazil seems to be high. Department of Veterinary Pathology School of Agrarian and Veterinary Sciences (FCAV/UNESP) Department of Veterinary Clinics and Surgery School of Agrarian and Veterinary Sciences (FCAV/UNESP) Department of Preventive Veterinary Medicine and Animal Reproduction School of Agrarian and Veterinary Sciences (FCAV/UNESP) Department of Veterinary Pathology School of Agrarian and Veterinary Sciences (FCAV/UNESP) Department of Veterinary Clinics and Surgery School of Agrarian and Veterinary Sciences (FCAV/UNESP) Department of Preventive Veterinary Medicine and Animal Reproduction School of Agrarian and Veterinary Sciences (FCAV/UNESP) |
| Databáze: | OpenAIRE |
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