ALTERED MERISTEM PROGRAM1 regulates leaf identity independent of miR156-mediated translational repression

Autor: R. Scott Poethig, Jim P. Fouracre, Victoria J. Chen
Rok vydání: 2020
Předmět:
Zdroj: Development
ISSN: 1477-9129
0950-1991
DOI: 10.1242/dev.186874
Popis: InArabidopsis, loss of the carboxypeptidase, ALTERED MERISTEM PROGRAM1 (AMP1), produces an increase in the rate of leaf initiation, an enlarged shoot apical meristem and an increase in the number of juvenile leaves. This phenotype is also observed in plants with reduced levels of miR156-targetedSQUAMOSA PROMOTER BINDING PROTEIN-LIKE(SPL) transcription factors, suggesting that AMP1 may promote SPL activity. However, we found that theamp1phenotype is only partially corrected by elevatedSPLgene expression, and thatamp1has no significant effect onSPLtranscript levels, or on the level or the activity of miR156. Although evidence from a previous study suggests that AMP1 promotes miRNA-mediated translational repression,amp1did not prevent the translational repression of the miR156 target,SPL9, or the miR159 target,MYB33.These results suggest thatAMP1regulates vegetative phase change downstream of, or in parallel to, the miR156/SPLpathway and that it is not universally required for miRNA-mediated translational repression.Summary statementWe show that loss of the carboxypeptidase, AMP1, does not interfere with the function of miR156 or miR159, suggesting that AMP1 is not universally required for miRNA-mediated translational repression inArabidopsis.
Databáze: OpenAIRE