Alpha‐keratin and corneous beta protein in the parakeratinized epithelium of the tongue in the domestic goose (Anser anser f. domestica)
| Autor: | Kinga Skieresz-Szewczyk, Tomasz Buchwald, Mirosław Szybowicz, Hanna Jackowiak |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Biology Spectrum Analysis Raman 010603 evolutionary biology 01 natural sciences Epithelium 03 medical and health sciences Basal (phylogenetics) Tongue Geese Keratin Genetics medicine Animals Ecology Evolution Behavior and Systematics chemistry.chemical_classification integumentary system beta-Keratins Immunohistochemistry Molecular biology Staining Corneous 030104 developmental biology medicine.anatomical_structure chemistry Nail (anatomy) Keratins Molecular Medicine Animal Science and Zoology Developmental Biology |
| Zdroj: | Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 332:158-166 |
| ISSN: | 1552-5015 1552-5007 |
| DOI: | 10.1002/jez.b.22892 |
| Popis: | The parakeratinized epithelium is a common epithelium in the oral cavity in birds and is characterized by the presence of cell nuclei in the cells of the cornified layer. This epithelium covers almost the entire dorsal surface of the tongue in the domestic goose apart of the lingual nail and conical papillae. So far no study has identified the molecular proteins alpha-keratin (IF-keratin) and/or corneous beta protein (CBP), which are responsible for keratinization or cornification processes in the parakeratinized epithelium of domestic geese. The study was performed using immunohistochemical (IHC) methods to identify alpha-keratin. The innovative method of Raman microspectroscopy was used to determine the presence of CBP and specify their percentage in epithelial layers of the parakeratinized epithelium. The results revealed that alpha-keratin is present in the whole parakeratinized epithelium. A strong staining reaction was detected in the basal and intermediate layers and a less strong staining reaction in the cornified layer. Raman microspectroscopy analysis confirmed the presence of alpha-keratin and demonstrated that its percentage decreases from the basal layer to the cornified layer. The Raman microspectroscopy technique revealed the occurrence of CBP in the parakeratinized epithelium and demonstrated that the percentage of this protein increases from the basal layer to the cornified layer. Performed analysis determines that parakeratinized epithelium undergoes cornification. However, the lower percentage of CBP in the cornified layer of parakeratinized epithelium than in orthokeratinized epithelium points to the fact that parakeratinized epithelium has a weaker protective function. |
| Databáze: | OpenAIRE |
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