Monoclonal antibody-mediated tumor regression by induction of apoptosis
| Autor: | Peter H. Krammer, C. Klas, Peter Möller, Anke M.J. Peters, Werner Falk, Klaus-Michael Debatin, B. C. Trauth, Siegfried Matzku |
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| Rok vydání: | 1989 |
| Předmět: |
Programmed cell death
medicine.drug_class Cell Survival T-Lymphocytes 610 Medizin Mice Nude Antibodies Monoclonal/therapeutic use Biology Monoclonal antibody Mice Antigen Leukemia B-Cell/therapy Antigens Neoplasm medicine Leukemia B-Cell Tumor Cells Cultured Animals Humans Fragmentation (cell biology) Cells Cultured Antigens Neoplasm/immunology B-Lymphocytes ddc:610 Burkitt Lymphoma/therapy Multidisciplinary Remission Induction Antibodies Monoclonal B-Lymphocytes/immunology T-Lymphocytes/immunology Fas receptor Burkitt Lymphoma Precipitin Tests Apoptosis Cell culture Immunology biology.protein Cancer research Autoradiography Electrophoresis Polyacrylamide Gel Antibody |
| DOI: | 10.5283/epub.16007 |
| Popis: | To characterize cell surface molecules involved in control of growth of malignant lymphocytes, monoclonal antibodies were raised against the human B lymphoblast cell line SKW6.4. One monoclonal antibody, anti-APO-1, reacted with a 52-kilodalton antigen (APO-1) on a set of activated human lymphocytes, on malignant human lymphocyte lines, and on some patient-derived leukemic cells. Nanogram quantities of anti-APO-1 completely blocked proliferation of cells bearing APO-1 in vitro in a manner characteristic of a process called programmed cell death or apoptosis. Cell death was preceded by changes in cell morphology and fragmentation of DNA. This process was distinct from antibody- and complement-dependent cell lysis and was mediated by the antibody alone. A single intravenous injection of anti-APO-1 into nu/nu mice carrying a xenotransplant of a human B cell tumor induced regression of this tumor within a few days. Histological thin sections of the regressing tumor showed that anti-APO-1 was able to induce apoptosis in vivo. Thus, induction of apoptosis as a consequence of a signal mediated through cell surface molecules like APO-1 may be a useful therapeutic approach in treatment of malignancy. |
| Databáze: | OpenAIRE |
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