Induction of lupus-related specific autoantibodies by non-specific inflammation caused by an intraperitoneal injection of n-hexadecane in BALB/c mice
| Autor: | Yoshioki Yamasaki, Jun Akaogi, Westley H. Reeves, Nobutaka Ono, Yoshiki Kuroda, Minoru Satoh, Dina C. Nacionales, Tolga Barker |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_treatment
Intraperitoneal injection Arthritis Autoimmunity Peritonitis Toxicology BALB/c Mice Squalene chemistry.chemical_compound Alkanes medicine Animals Autoantibodies Lupus Vulgaris Mice Inbred BALB C Systemic lupus erythematosus biology Pristane Autoantibody Ascites biology.organism_classification medicine.disease Molecular biology Liver chemistry Immunology Environmental Pollutants Female Adjuvant Injections Intraperitoneal Spleen |
| Zdroj: | Toxicology. 218:186-196 |
| ISSN: | 0300-483X |
| Popis: | A single intraperitoneal (i.p.) injection of pristane, incomplete Freund's adjuvant (IFA), or the adjuvant oil squalene, but not high molecular weight medicinal mineral oils, induces lupus-related autoantibodies to nRNP/Sm and -Su in non-autoimmune strains of mice. This ability appears to be associated with the low molecular weight and adjuvanticity of hydrocarbon. n-Hexadecane (C(16)H(34)), which is present in petroleum, has adjuvant activity and induces arthritis in rodents like other lupus-inducing oils. In addition to dietary exposure to n-hexadecane in mineral oils, exposure also occurs via inhalation of oil mist, jet fuel, or diesel exhaust or by absorption through the skin. Since n-hexadecane is a low molecular weight adjuvant hydrocarbon oil similar to other lupus-inducing hydrocarbons, the present study examined whether it can also induce lupus-related autoantibodies in mice. Female BALB/cJ mice received a single i.p. injection of 0.5 ml of n-hexadecane, pristane, or saline (control). Pathology and serology (immunoglobulin levels, autoantibodies by immunofluorescence, immunoprecipitation, and ELISA) were examined 3 months later. Unexpectedly, all n-hexadecane-treated mice, but none in the other groups, developed inflammatory ascites within 2.5 months. n-Hexadecane induced hypergammaglobulinemia (IgG1, IgG2a), antinuclear (titer>1:160, 67%) and -cytoplasmic antibodies (58%) and autoantibodies to nRNP/Sm (25%), Su (33%), ssDNA (83%), and chromatin (100%). Therefore, non-specific inflammation caused by n-hexadecane resulted in the production of a limited set of specific autoantibodies. These previously unrecognized immunological effects of n-hexadecane may have implications in monitoring human exposure to hydrocarbons and in the pathogenesis of autoimmune diseases. |
| Databáze: | OpenAIRE |
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