Comparative analysis of cellulose acetate hemoglobin electrophoresis and high performance liquid chromatography for quantitative determination of hemoglobin A2
| Autor: | Hassan Osman Mehmood, Khansa Qamar, Moinuddin Moinuddin, Shafi Mohammad Khosa, Muhammad Usman |
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| Rok vydání: | 2015 |
| Předmět: |
Chromatography
Hemoglobin electrophoresis Cost effectiveness business.industry Hematology Cellulose acetate hemoglobin electrophoresis Bioinformatics High-performance liquid chromatography Cellulose acetate Quantitative determination Electrophoresis chemistry.chemical_compound chemistry Hemoglobin A2 Medicine Original Article business Reliability (statistics) High performance liquid chromatography |
| Zdroj: | Blood research |
| ISSN: | 2288-0011 2287-979X |
| DOI: | 10.5045/br.2015.50.1.46 |
| Popis: | Background The present study is designed to evaluate the reliability and cost effectiveness of cellulose acetate Hb electrophoresis and high performance liquid chromatography (HPLC) in the determination of HbA2 levels. Methods The test population comprised 160 individuals divided into four groups: normal individuals, β-thalassemia trait (BTT) patients, iron deficiency anemia (IDA) patients, and co-morbid patients (BTT with IDA). HbA2 levels determined using cellulose acetate Hb electrophoresis and HPLC were compared. Results HbA2 levels were found to be diagnostic for classical BTT using either method. In co-morbid cases, both techniques failed to diagnose all cases of BTT. The sensitivity, specificity, and Youden's index for detection of the co-morbid condition was 69% and 66% for HPLC and cellulose acetate Hb electrophoresis, respectively. Conclusion This study revealed that semi-automated cellulose acetate Hb electrophoresis is more suitable for use in β-thalassemia prevention programs in low-income countries like Pakistan. This technique is easily available, simple and cost effective. |
| Databáze: | OpenAIRE |
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