Spatial dynamics and mixing of bluefin tuna in the Atlantic Ocean and Mediterranean Sea revealed using next generation sequencing
| Autor: | Piero Addis, Gregory E. Maes, Andone Estonba, Gregory Neils Puncher, Haritz Arrizabalaga, Işık Oray, Molly Lutcavage, S. Zgozi, Francisco Alemany, Noureddine Abid, Igaratza Fraile, Naiara Rodríguez-Ezpeleta, Firdes Karakulak, Gualtiero Basilone, Fulvio Garibaldi, Fausto Tinti, Nicolas Goñi, Joseph M. Quattro, Koen Herten, Ai Kimoto, James S. Franks, David Macías, Urtzi Laconcha, Simeon Deguara, Jeroen Van Houdt, Rita Cannas, Alessia Cariani, Miguel N. Santos, Alex Hanke, Jay R. Rooker, Aitor Albaina |
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| Přispěvatelé: | European Commission, Ministerio de Ciencia e Innovación (España), Puncher, Gregory N, Cariani, Alessia, Maes, Gregory E, Van Houdt, Jeroen, Herten, Koen, Cannas, Rita, Rodriguez-Ezpeleta, Naiara, Albaina, Aitor, Estonba, M Andone, Lutcavage, Molly, Hanke, Alex, Rooker, Jay, Franks, James S, Quattro, Joseph M, Basilone, Gualtiero, Fraile, Igaratza, Laconcha, Urtzi, Goñi, Nicola, Kimoto, Ai, Macías, A David, Alemany, Francisco, Deguara, Simeon, Zgozi, Salem W, Garibaldi, Fulvio, Oray, Isik K, Karakulak, F Saadet, Abid, Noureddine, Santos, Miguel N, Addis, Piero, Arrizabalaga, Haritz, Tinti, Fausto |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Reduced Representation Sequencing Genotyping Techniques origin assignment Single Nucleotide Polymorphisms Population Dynamics Population Sede Central IEO Biology Polymorphism Single Nucleotide DNA sequencing 03 medical and health sciences Mediterranean sea Gene Frequency Effective population size Mediterranean Sea Genetics Animals Pesquerías mixed stock analysi education Atlantic Ocean Allele frequency Genotyping Ecology Evolution Behavior and Systematics education.field_of_study Tuna Mixed-stock analysis Chromosome Mapping population structure mixed stock analysis Thunnus thynnu Sequence Analysis DNA 030104 developmental biology Evolutionary biology Single Nucleotide Polymorphism Animal Migration Thunnus thynnus Fisheries management Biotechnology |
| Popis: | The Atlantic bluefin tuna is a highly migratory species emblematic of the challenges associated with shared fisheries management. In an effort to resolve the species' stock dynamics, a genomewide search for spatially informative single nucleotide polymorphisms (SNPs) was undertaken, by way of sequencing reduced representation libraries. An allele frequency approach to SNP discovery was used, combining the data of 555 larvae and young-of-the-year (LYOY) into pools representing major geographical areas and mapping against a newly assembled genomic reference. From a set of 184,895 candidate loci, 384 were selected for validation using 167 LYOY. A highly discriminatory genotyping panel of 95 SNPs was ultimately developed by selecting loci with the most pronounced differences between western Atlantic and Mediterranean Sea LYOY. The panel was evaluated by genotyping a different set of LYOY (n = 326), and from these, 77.8% and 82.1% were correctly assigned to western Atlantic and Mediterranean Sea origins, respectively. The panel revealed temporally persistent differentiation among LYOY from the western Atlantic and Mediterranean Sea (FST = 0.008, p = .034). The composition of six mixed feeding aggregations in the Atlantic Ocean and Mediterranean Sea was characterized using genotypes from medium (n = 184) and large (n = 48) adults, applying population assignment and mixture analyses. The results provide evidence of persistent population structuring across broad geographic areas and extensive mixing in the Atlantic Ocean, particularly in the mid-Atlantic Bight and Gulf of St. Lawrence. The genomic reference and genotyping tools presented here constitute novel resources useful for future research and conservation efforts. This work was carried out under the provision of the ICCAT Atlantic-wide Research Programme for Bluefin Tuna (GBYP), funded by the European Union, by several ICCAT CPCs, the ICCAT Secretariat and other entities (see: http://www.iccat.int/GBYP/en/Budget.htm). Additional funds were provided by the MARES Joint Doctorate Programme Selected Under Erasmus Mundus and coordinated by Ghent University (FPA 2011-0016). Larvae from the Balearic Sea were provided by the ATAME project “Bluefin tuna abundance indices: towards recruitment estimators based on larval ecology” (CTM 2011-29525-C04-02) and the BLUEFIN project “Modelling bluefin spawning areas variability and population dynamics in the Western Mediterranean” (IEO-SOCIB agreement). |
| Databáze: | OpenAIRE |
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