Protocadherin 12 (VE-cadherin 2) is expressed in endothelial, trophoblast, and mesangial cells
| Autor: | Emmanuelle Tillet, Muriel Vernet, Hervé Pointu, Marie-Hélène Prandini, Stéphanie Bouillot, Philippe Huber, Christine Rampon, Ronald Frank |
|---|---|
| Přispěvatelé: | Laboratoire de développement et vieillissement de l'endothélium, Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF), Atelier de Transgenèse, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Department of Chemical Biology, German Research Centre for Biotechnology, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Huber, Philippe |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Male
MESH: Trophoblasts Cellular differentiation MESH: Glycogen MESH: Mice Knockout MESH: Cadherins Mice 0302 clinical medicine MESH: Gene Expression Regulation Developmental MESH: Animals MESH: Endothelial Cells Mice Knockout 0303 health sciences Cell adhesion molecule Gene Expression Regulation Developmental Cell Differentiation Cadherins Glomerular Mesangium Trophoblasts medicine.anatomical_structure MESH: Pluripotent Stem Cells Female Glycogen Pluripotent Stem Cells MESH: Cell Differentiation Endothelium MESH: Glomerular Mesangium Protocadherin [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Cell Line 03 medical and health sciences medicine Animals [SDV.BC] Life Sciences [q-bio]/Cellular Biology MESH: Mice 030304 developmental biology Cadherin MESH: Biological Markers Trophoblast Endothelial Cells Cell Biology Embryonic stem cell Molecular biology Protocadherins MESH: Male MESH: Cell Line VE-cadherin MESH: Female 030217 neurology & neurosurgery Biomarkers |
| Zdroj: | Experimental Cell Research Experimental Cell Research, Elsevier, 2005, 302 (1), pp.48-60. ⟨10.1016/j.yexcr.2004.08.024⟩ Experimental Cell Research, 2005, 302 (1), pp.48-60. ⟨10.1016/j.yexcr.2004.08.024⟩ |
| ISSN: | 0014-4827 1090-2422 |
| DOI: | 10.1016/j.yexcr.2004.08.024⟩ |
| Popis: | International audience; Protocadherin 12 protein (PCDH12, VE-cadherin 2) is a cell adhesion molecule that has been isolated from endothelial cells. Here, we have used Northern and Western blots, immunohistology, and flow cytometry to examine the distribution of PCDH12 in mouse tissues. It is an N-glycosylated protein of 150-kDa mass. In the endothelium, PCDH12 immunoreactivity was variable and dependent upon the vascular bed. In both the embryo and embryonic stem cell differentiation system, signals were localized in vasculogenic rather than angiogenic endothelium. In addition, the protein was strongly expressed in a subset of invasive cells of the placenta, which were identified as glycogen-rich trophoblasts. In adult mice, strong PCDH12 signals were observed in mesangial cells of kidney glomeruli whereas expression was not detected in other types of perivascular cells. As opposed to most protocadherins, PCDH12 is not expressed in early embryonic (day 12.5) and adult brains. As a first approach to obtain insight into PCDH12 function, we produced transgenic mice deficient in PCDH12, which were viable and fertile. They did not display any obvious histomorphological defects. We conclude that PCDH12 has a unique expression pattern and that its deficiency does not lead to conspicuous abnormalities. Moreover, PCDH12 is the first specific marker for both glycogen-rich trophoblasts and mesangial cells. |
| Databáze: | OpenAIRE |
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