Diatom-derived oxylipins induce cell death in sea urchin embryos activating caspase-8 and caspase 3/7
| Autor: | Nadia Ruocco, Stefano Mellone, Maria Costantini, Stefano Varrella, Antonio Leonardi, Adrianna Ianora, Giovanna Romano, Domenico Somma, Matt G. Bentley, Antonio Zuppa |
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| Přispěvatelé: | Ruocco, Nadia, Varrella, Stefano, Romano, Giovanna, Ianora, Adrianna, Bentley, Matt G., Somma, Domenico, Leonardi, Antonio, Mellone, Stefano, Zuppa, Antonio, Costantini, MARIA PAOLA |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Sea urchin Programmed cell death Embryo Nonmammalian Health Toxicology and Mutagenesis Apoptosis Caspase 3 010501 environmental sciences Aquatic Science Caspase 8 01 natural sciences Paracentrotus lividus 03 medical and health sciences chemistry.chemical_compound biology.animal Botany Animals Oxylipins 14. Life underwater Caspase 0105 earth and related environmental sciences Diatoms HEPES biology Medicine (all) Apoptosi Diatom biology.organism_classification 030104 developmental biology Biochemistry chemistry Caspases Paracentrotus biology.protein Water Pollutants Chemical |
| Zdroj: | Aquatic toxicology 176 (2016): 128–140. doi:10.1016/j.aquatox.2016.04.012 info:cnr-pdr/source/autori:Ruocco N.; Varrella S.; Romano G.; Ianora A.; Bentley M.G.; Somma D.; Leonardi A.; Mellone S.; Zuppa A.; Costantini M./titolo:Diatom-derived oxylipins induce cell death in sea urchin embryos activating caspase-8 and caspase 3%2F7/doi:10.1016%2Fj.aquatox.2016.04.012/rivista:Aquatic toxicology/anno:2016/pagina_da:128/pagina_a:140/intervallo_pagine:128–140/volume:176 |
| ISSN: | 0166-445X |
| Popis: | Diatoms are an important class of unicellular algae that produce bioactive secondary metabolites with cytotoxic activity collectively termed oxylipins, including polyunsaturated aldehydes (PUAs), hydroxyacids (HEPEs), oxo-acids and epoxyalcohols. Previous results showed that at higher concentrations, the PUA decadienal induced apoptosis on copepods and sea urchin embryos via caspase-3 activation; at lower concentrations decadienal affected the expression levels of the caspase-8 gene in embryos of the sea urchin Paracentrotus lividus. In the present work, we studied the effects of other common oxylipins produced by diatoms: two PUAs (heptadienal and octadienal) and four hydroxyacids (5-, 9- 11- and 15-HEPE) on P. lividus cell death and caspase activities. Our results showed that (i) at higher concentrations PUAs and HEPEs induced apoptosis in sea urchin embryos, detected by microscopic observation and through the activation of caspase-3/7 and caspase-8 measured by luminescent assays; (ii) at low concentrations, PUAs and HEPEs affected the expression levels of caspase-8 and caspase-3/7 (isolated for the first time here in P. lividus) genes, detected by Real Time qPCR. These findings have interesting implications from the ecological point of view, given the importance of diatom blooms in nutrient-rich aquatic environments. |
| Databáze: | OpenAIRE |
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