Optimization of 3-D hepatocyte culture by controlling the physical and chemical properties of the extra-cellular matrices
| Autor: | Yingnan Wu, Jian-Hang Zhu, Susanne Ng, Hanry Yu, Zi-Zong Ho, Yier Toh, Yi Zhou, Ser-Mien Chia, Hai-Quan Mao |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Materials science Polymers Cell Culture Techniques Biophysics Bioengineering 7-Alkoxycoumarin O-Dealkylase Methylmethacrylate Methylation law.invention Biomaterials Confocal microscopy law Image Processing Computer-Assisted medicine Animals Scattering Radiation Rats Wistar Cells Cultured Chromatography High Pressure Liquid Microscopy Confocal Microcirculation Galactose In vitro Extracellular Matrix Rats medicine.anatomical_structure Models Chemical Biochemistry Mechanics of Materials Cell culture Hepatocyte Hepatocytes Ceramics and Composites Methacrylates Asialoglycoprotein receptor Collagen |
| Zdroj: | Biomaterials. 26:3153-3163 |
| ISSN: | 0142-9612 |
| Popis: | Hepatocytes are anchorage-dependent cells sensitive to microenvironment; the control of the physicochemical properties of the extra-cellular matrices may be useful to the maintenance of hepatocyte functions in vitro for various applications. In a microcapsule-based 3-D hepatocyte culture microenvironment, we could control the physical properties of the collagen nano-fibres by fine-tuning the complex-coacervation reaction between methylated collagen and terpolymer of hydroxylethyl methacrylate-methyl methacrylate-methylacrylic acid. The physical properties of the nano-fibres were quantitatively characterized using back-scattering confocal microscopy to help optimize the physical support for hepatocyte functions. We further enhanced the chemical properties of the collagen nano-fibres by incorporating galactose onto collagen, which can specifically interact with the asialoglycoprotein receptor on hepatocytes. By correlating a range of collagen nano-fibres of different physicochemical properties with hepatocyte functions, we have identified a specific combination of methylated and galactosylated collagen nano-fibres optimal for maintaining hepatocyte functions in vitro. A model of how the physical and chemical supports interplay to maintain hepatocyte functions is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect