Stat3-Atg5 signal axis inducing autophagy to alleviate hepatic ischemia-reperfusion injury
| Autor: | Yan‐bing Zhao, Jun Li, Shi‐peng Li, Li Li, Yu‐fang Han, Zhong‐dong Li, Yong‐gan Li |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male STAT3 Transcription Factor Cell Survival ATG5 Blotting Western Apoptosis Biochemistry Autophagy-Related Protein 5 Cell Line 03 medical and health sciences Mice 0302 clinical medicine Microscopy Electron Transmission medicine Autophagy Animals STAT3 Molecular Biology Microscopy Confocal biology Cell Biology medicine.disease Immunohistochemistry Cell biology Mice Inbred C57BL Disease Models Animal 030104 developmental biology Liver 030220 oncology & carcinogenesis Reperfusion Injury Immunology STAT protein biology.protein Signal transduction Reperfusion injury Signal Transduction |
| Zdroj: | Journal of cellular biochemistry. 119(4) |
| ISSN: | 1097-4644 |
| Popis: | In performing our experiment, impaired autophagy increased hepatocellular damage during the reperfusion period. It was demonstrated by the effect of blocking autophagy using bafilomycin A1 or knocking Atg5 gene out reduces the anti-apoptotic effect of Stat3. Here we focus on the role of signal transducer and activator of transcription 3 (Stat3) in regulating autophagy to alleviate hepatic IRI. We found that Stat3 was up-regulated during hepatic IRI and was associated with an activation of the autophagic signaling pathway. This increased Stat3 expression, which was allied with high autophagic activity, alleviated liver damage to IR, an effect which was abrogated by Stat3 epletion as demonstrated in both in vivo and in vitro methods. The levels of Atg5 protein were decreased when Stat3 was inhibited by HO 3867 or siStat3. We conclude that Stat3 appeared to exert a pivotal role in hepatic IRI, by activating autophagy to alleviate hepatic IRI, and Atg5 was required for this process. The identification of this novel pathway, that links expression levels of Stat3 with Atg5-mediated autophagy, may provide new insights for the generation of novel protective therapies directed against hepatic IRI. |
| Databáze: | OpenAIRE |
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