Methodological Guidelines for Genetic Safety Testing of Cell Transplants
| Autor: | V. A. Nikitina, N. P. Kuleshov, N. P. Bochkov, E. S. Voronina |
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| Rok vydání: | 2009 |
| Předmět: |
Cell
Cell Culture Techniques Multipotent Mesenchymal Stromal Cells Aneuploidy Guidelines as Topic Computational biology In situ hybridization Biology Mesenchymal Stem Cell Transplantation General Biochemistry Genetics and Molecular Biology Cytogenetics medicine Humans Safety testing Cells Cultured In Situ Hybridization Fluorescence Ploidies Multipotent Stem Cells Karyotype General Medicine medicine.disease Cell biology medicine.anatomical_structure Karyotyping Interphase Stromal Cells Stem cell |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 148:677-683 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-010-0793-7 |
| Popis: | A combination of karyotyping and aneuploidy analysis by interphase fluorescent in situ hybridization is a sensitive method for evaluation of genetic stability of stem cell cultures. The methodology and specific features of preparing and analyzing the cytogenetic preparations are described as exemplified by human multipotent mesenchymal stromal cells. |
| Databáze: | OpenAIRE |
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