Regeneration of transgenic cassava plants (Manihot esculenta Crantz) through Agrobacterium-mediated transformation of embryogenic suspension cultures

Autor:	A. E. González, Roger N. Beachy, Claude M. Fauquet, C. Schöpke, Nigel J. Taylor
Rok vydání:	2019
Předmět:	Rhizobiaceae biology Agrobacterium fungi food and beverages Plant Science General Medicine Agrobacterium tumefaciens biology.organism_classification Transformation (genetics) Tissue culture Callus Botany Agronomy and Crop Science Selectable marker Southern blot
Zdroj:	Plant cell reports. 17(11)
ISSN:	1432-203X
Popis:	A protocol was developed for Agrobacterium-mediated transformation of embryogenic suspension cultures of cassava. The bacterial strain ABI containing the binary vector pMON977 with the nptII gene as selectable marker and an intron-interrupted uidA gene (encoding β-glucuronidase) as visible marker was used for the experiments. Selection of transformed tissue with paromomycin resulted in the establishment of antibiotic-resistant, β-glucuronidase-expressing lines of friable embryogenic callus, from which embryos and subsequently plants were regenerated. Southern blot analysis demonstrated stable integration of the uidA gene into the cassava genome in five lines of transformed embryogenic suspension cultures and in two plant lines.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15261404a359e6055c6b5cc1a655f40d https://pubmed.ncbi.nlm.nih.gov/30736551 Zobrazit plný text záznamu Full text from SpringerLink