The effects on steroidogenesis and histopathology of adult male Japanese quails (Coturnix coturnix japonica) testis following pre-pubertal exposure to di(n-butyl) phthalate (DBP)
| Autor: | Hermanus B. Groenewald, Umar Muhammed Bello, Augustine Arukwe, Tom A. Aire, Mary-Catherine Madekurozwa |
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| Rok vydání: | 2014 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Physiology Health Toxicology and Mutagenesis Coturnix Endocrine Disruptors Biology Toxicology Biochemistry Avian Proteins Random Allocation chemistry.chemical_compound Hormesis Plasticizers Internal medicine Testis medicine Animals Endocrine system Cholesterol Side-Chain Cleavage Enzyme Spermatogenesis Testosterone Dose-Response Relationship Drug Leydig cell Bird Diseases Cholesterol side-chain cleavage enzyme Hydroxysteroid Dehydrogenases Phthalate Gene Expression Regulation Developmental Leydig Cells Reproducibility of Results Radioimmunoassay Organ Size Cell Biology General Medicine Phosphoproteins Dibutyl Phthalate medicine.anatomical_structure Endocrinology chemistry Infertility Histopathology Atrophy |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 166:24-33 |
| ISSN: | 1532-0456 |
| Popis: | In the present study, we have investigated the effects of 30-day dietary (pre-pubertal) exposure to different doses (0 (control), 1, 10, 50, 200 and 400 mg/kg bodyweight/day) of di(n-butyl) phthalate (DBP) on Leydig cells of adult male Japanese quails by quantifying the transcript levels for P450 side-chain cleavage (p450scc), P450c17 (CYP17), and 3β- and 17β-hydroxysteroid dehydrogenase (hsd) using quantitative (real-time) polymerase chain reaction (qRT-PCR). In addition, the plasma testosterone levels were analysed using radioimmunoassay (RIA) and testis was examined for evidence of gross pathology and histopathology. Our data showed that pre-pubertal exposure to DBP produced alterations in testicular architecture as evident by poorly developed or mis-shaped testis, and altered spermatogenesis due to tubular degeneration and atrophy of seminiferous tubules especially in the high DBP dose (200 and 400 mg/kg) treated groups. In addition, DBP altered several key enzymes involved in testicular steroidogenesis pathways in an apparent dose-dependent manner. For example, biphasic effects of DBP were observed for P450scc and 3β-hsd mRNA, that were generally increasing at low dose 10 mg/kg, and thereafter, an apparent dose-dependent decrease between 50 and 400mg/kg. The steroidogenic acute regulatory (StAR) protein was at the lowest detectable limits and therefore not quantifiable. These effects did not parallel the non-significant changes observed for plasma testosterone levels. The present data is consistent with previous reports showing that DBP modulates Leydig cell steroidogenesis in several species, with a potential negative effect on reproduction in those avian species that are vulnerable to endocrine disrupting chemicals. |
| Databáze: | OpenAIRE |
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