In Vitro Propagation of Rubber Tree (Hevea Brasiliensis) Using Shoot-Tip and Nodal Cutting Explants
| Autor: | George Y. P. Klu, Rhoda Gyinae Diawuoh, Anthony Antwi-Wiredu, Samuel Amiteye, Alex Kofi Asumeng |
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| Rok vydání: | 2018 |
| Předmět: |
biology
Euphorbiaceae food and beverages biology.organism_classification Horticulture chemistry.chemical_compound Murashige and Skoog medium Natural rubber chemistry Micropropagation Hevea brasiliensis In Vitro Propagation Shoot-Tip and Nodal Culture MS Medium Kinetin visual_art Shoot visual_art.visual_art_medium Kinetin Hevea brasiliensis Explant culture |
| Zdroj: | I. J. of Advances in Scientific Research and Engineering-IJASRE (ISSN: 2454-8006); Vol. 4 No. 6` (2018): Volume 4 Issue 6 June (2018); 38-50 |
| ISSN: | 2454-8006 |
| Popis: | Hevea brasiliensis which belongs to the Euphorbiaceae family is the primary source of natural rubber. Propagation of rubber tree by grafting on to unselected seedlings sustains intraclonal heterogeneity for vigour and productivity which could be improved via in vitro techniques. Micropropagation from rubber nodal and shoot tip explants is possible. In vitro technique is needful to mass propagate disease-free and genetically similar rubber plantlets. In vitro results in increased growth and vigour of rubber tree, However, in vitro techniques of rubber tree have not been given much critical research attention in Ghana. Propagation of H. brasiliensis by in vitro techniques was used to study alternative procedures for mass production of rubber planting materials. Murashige and Skoog (MS) basal medium amended with 30.0g/L sucrose, 100.0mg/L myo-inositol, 2.0g/L activated charcoal, 1.0mg/L silver nitrate AgNO3, 2.0mg/L GA3 and control, 2.5, 5.0, 7.5 or 10.0mg/L kinetin was used to culture both H. brasiliensis shoottip and nodal explants. The MS medium with 5.0mg/L kinetin significantly (P |
| Databáze: | OpenAIRE |
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