Zinc Fingers in HIV-1 Gag Precursor Are Not Equivalent for gRNA Recruitment at the Plasma Membrane
| Autor: | Pascal Didier, Raphael Cathagne, Hugues de Rocquigny, Maaz Bin Nasim, Jeremy Bonzi, Roland Marquet, Yves Mély, Philippe Carl, Denis Dujardin, Serena Bernacchi, Halina Anton, Eléonore Réal, Jean-Christophe Paillart, Emmanuel Boutant |
|---|---|
| Přispěvatelé: | Laboratoire de Bioimagerie et Pathologies (LBP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Architecture et Réactivité de l'ARN (ARN), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Morphogénèse et antigénicité du VIH et du virus des Hépatites (MAVIVH - U1259 Inserm - CHRU Tours ), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours, MATHELIN, Sandra |
| Rok vydání: | 2020 |
| Předmět: |
[SDV]Life Sciences [q-bio]
viruses Mutant Biophysics [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology 03 medical and health sciences 0302 clinical medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Guide RNA 030304 developmental biology Ribonucleoprotein Zinc finger 0303 health sciences Chemistry Virus Assembly Cell Membrane [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Zinc Fingers Genomics Articles 3. Good health Cell biology [SDV] Life Sciences [q-bio] Cytosol Membrane Cytoplasm HIV-1 RNA Viral 030217 neurology & neurosurgery Intracellular RNA Guide Kinetoplastida |
| Zdroj: | Biophys J Biophysical Journal Biophysical Journal, Biophysical Society, 2020, 119 (2), pp.419-433. ⟨10.1016/j.bpj.2020.05.035⟩ Biophysical Journal, 2020, 119 (2), pp.419-433. ⟨10.1016/j.bpj.2020.05.035⟩ |
| ISSN: | 1542-0086 0006-3495 |
| Popis: | International audience; The human immunodeficiency virus type 1 Gag precursor specifically selects the unspliced viral genomic RNA (gRNA) from the bulk of cellular and spliced viral RNAs via its nucleocapsid (NC) domain and drives gRNA encapsidation at the plasma membrane (PM). To further identify the determinants governing the intracellular trafficking of Gag-gRNA complexes and their accumulation at the PM, we compared, in living and fixed cells, the interactions between gRNA and wild-type Gag or Gag mutants carrying deletions in NC zinc fingers (ZFs) or a nonmyristoylated version of Gag. Our data showed that the deletion of both ZFs simultaneously or the complete NC domain completely abolished intracytoplasmic Gag-gRNA interactions. Deletion of either ZF delayed the delivery of gRNA to the PM but did not prevent Gag-gRNA interactions in the cytoplasm, indicating that the two ZFs display redundant roles in this respect. However, ZF2 played a more prominent role than ZF1 in the accumulation of the ribonucleoprotein complexes at the PM. Finally, the myristate group, which is mandatory for anchoring the complexes at the PM, was found to be dispensable for the association of Gag with the gRNA in the cytosol. |
| Databáze: | OpenAIRE |
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