Development of a method based on surface enhanced laser desorption and ionization time of flight mass spectrometry for rapid identification of Klebsiella pneumoniae
| Autor: | Hua Yu, Yongchang Yang, Bo Huang, Dai-Wen Xiao, Qi Hu, Weijin Liao, Hua Liu, Wei Jiang, Yingjun Yan, Wen-Fang Huang |
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| Rok vydání: | 2009 |
| Předmět: |
Bacteriological Techniques
Chromatography biology Klebsiella pneumoniae Chemistry K pneumoniae General Medicine Mass spectrometry Laser biology.organism_classification Applied Microbiology and Biotechnology Microbiology Sensitivity and Specificity Mass Spectrometry law.invention Klebsiella Infections Rapid identification law SELDI-TOF-MS Desorption Ionization time of flight Humans Neural Networks Computer |
| Zdroj: | Journal of microbiology (Seoul, Korea). 47(5) |
| ISSN: | 1976-3794 |
| Popis: | A method based on surface enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOF MS) was developed for the rapid identification of Klebsiella pneumoniae by directly applying bacterial colonies without further protein extraction. A total of 40 K. pneumoniae and 114 other related microorganisms isolated clinically were analyzed by SELDI-TOF MS. An identification model for K. pneumoniae was established by artificial neural networks (ANNs) with classification accuracy of 100%. The model was blindly tested with 43 K. pneumoniae and 53 control bacteria again. The results showed that the model was successful with accuracy of 96.9%, sensitivity of 100% and specificity of 943%. This strategy is potential for rapid identification of K. pneumoniae. |
| Databáze: | OpenAIRE |
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