[Identifying the fluorescent body (F-body) with quinacrine mustard staining of canine]
| Autor: | Kazuhisa Tanaka, Shyoso Ogawa, Kiyonori Tauchi, Tsutomu Takeshima, Yo Igarashi |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Male
Lymphocyte Quinacrine Mustard General Biochemistry Genetics and Molecular Biology Fluorescence Dogs Y Chromosome Dispase medicine Fluorescence microscope Animals Lymphocytes Interphase Whole blood General Veterinary Spermatozoon Staining and Labeling Chemistry General Medicine Sperm Molecular biology Spermatozoa Staining medicine.anatomical_structure Quinacrine Animal Science and Zoology Female |
| Zdroj: | Jikken dobutsu. Experimental animals. 38(3) |
| ISSN: | 0007-5124 |
| Popis: | The fluorescent body (F-body) was identified with quinacrine mustard (Q-M) staining in spermatozoon and lymphocyte of canine. Well washed sperm suspension was treated with protease (125 mg/ml) or dispase (2000p. u./ml) and staining with Q-M (final dilution 50 micrograms/ml) for 15 min to 24 hr at 37 degrees C. The lymphocyte cultures from whole blood were prepared as routine human investigation. The chromosomal preparation made by air dry method was stained with Q-M (final dilution 0.5 to 50 micrograms/ml) after pretreatment of enzyme digestion. The examination using a reflected fluorescent microscope revealed that the same F-body in human was present in both spermatozoon (20.1-39.7%) and interphase of lymphocyte (0.37.2%) of male origin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|