Determination of Clobazam and Its Major Metabolite N-desmethylclobazam in Human Plasma with High-Performance Liquid Chromatography
| Autor: | Sherif Hanafy Mahmoud, Fadumo Ahmed Isse |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Analyte
QD71-142 Chromatography Clobazam medicine.diagnostic_test Chemistry Coefficient of variation Metabolite clobazam General Medicine 030226 pharmacology & pharmacy High-performance liquid chromatography 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pharmacokinetics Therapeutic drug monitoring medicine high-performance liquid chromatography Analytical chemistry plasma 030217 neurology & neurosurgery Active metabolite desmethylclobazam medicine.drug |
| Zdroj: | Analytica, Vol 2, Iss 7, Pp 57-65 (2021) Analytica Volume 2 Issue 3 Pages 7-65 |
| ISSN: | 2673-4532 |
| DOI: | 10.3390/analytica2030007 |
| Popis: | Clobazam (CLB) is a benzodiazepine that is used in many types of epilepsy. Although therapeutic drug monitoring (TDM) of CLB is not routine, there is evidence that TDM may be of value in conditions where pharmacokinetic alterations are suspected. Therefore, determination of both CLB and its active metabolite concentrations is essential for TDM. Herein, we present a simple and practical method for determination of CLB and N-desmethylclobazam (NDMCLB) in human plasma by high-performance liquid chromatography (HPLC). The drugs were extracted by hexane:dichloromethane (1:1, v/v) from 0.3 mL plasma. The separation was carried out with a C18 reverse phase column using a mobile phase of water:acetonitrile (57:43, v/v) pumped at 0.8 mL/min. The analytes were detected at 228 nm. The method was linear over the concentration range 20–500 ng/mL for CLB and 200–3000 ng/mL for NDMCLB. The intra-day coefficient of variation (CV) was < 10% for CLB and < 6% for NDMCLB, while the inter-day CV for CLB was < 16%. The metabolite inter-day CV was < 6%. The accuracy of intra- and inter-day assessments determined for CLB and NDMCLB was within ±10%. This paper describes a rapid, reliable, and simple method for measuring CLB and its metabolite NDMCLB in human plasma. This UV-HPLC procedure offers acceptable precision and accuracy to quantify CLB and its metabolite in human plasma. |
| Databáze: | OpenAIRE |
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