Pedigree simulations reveal that maternity assignment is reliable in populations with conspecific brood parasitism, incomplete parental sampling and kin structure
| Autor: | Bruce E. Lyon, John M. Eadie, Caroline M. Thow, Caitlin P. Wells |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Future studies Offspring Zoology Pedigree chart 010603 evolutionary biology 01 natural sciences Nesting Behavior 03 medical and health sciences Pregnancy Genetics Animals Allele frequency Ecology Evolution Behavior and Systematics 030304 developmental biology Retrospective Studies Brood parasite 0303 health sciences Cervus biology Sampling (statistics) Reproducibility of Results biology.organism_classification Pedigree Ducks Aix sponsa Female Biotechnology Microsatellite Repeats |
| Zdroj: | Molecular ecology resourcesREFERENCES. 22(1) |
| ISSN: | 1755-0998 |
| Popis: | Modern genetic parentage methods reveal that alternative reproductive strategies are common in both males and females. Under ideal conditions, genetic methods accurately connect the parents to offspring produced by extra-pair matings or conspecific brood parasitism. However, some breeding systems and sampling scenarios present significant complications for accurate parentage assignment. We used simulated genetic pedigrees to assess the reliability of parentage assignment for a series of challenging sampling regimes that reflect realistic conditions for many brood-parasitic birds: absence of genetic samples from sires, absence of samples from brood parasites and female kin-structured populations. Using 18 microsatellite markers and empirical allele frequencies from two populations of a conspecific brood parasite, the wood duck (Aix sponsa), we simulated brood parasitism and determined maternity using two widely used programs, cervus and colony. Errors in assignment were generally modest for most sampling scenarios but differed by program: cervus suffered from false assignment of parasitic offspring, whereas colony sometimes failed to assign offspring to their known mothers. Notably, colony was able to accurately infer unsampled parents. Reducing the number of markers (nine loci rather than 18) caused the assignment error to slightly worsen with colony but balloon with cervus. One potential error with important biological implications was rare in all cases-few nesting females were incorrectly excluded as the mother of their own offspring, an error that could falsely indicate brood parasitism. We consider the implications of our findings for both a retrospective assessment of previous studies and suggestions for best practices for future studies. |
| Databáze: | OpenAIRE |
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