Epstein-Barr virus nuclear antigen 3A protein regulates CDKN2B transcription via interaction with MIZ-1

Autor: Vincent Lotteau, Bettina Kempkes, Henri Gruffat, Pascal Leblanc, Maha Siouda, Quentin Bazot, Lionel Tafforeau, Chantal Rabourdin-Combe, Evelyne Manet, Massimo Tommasino, Marie L. Harth-Hertle, Thibaut Deschamps
Přispěvatelé: Manet, Evelyne, Herpesvirus oncogènes – Oncogenic Herpesviruses, Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre International de Recherche contre le Cancer - International Agency for Research on Cancer (CIRC - IARC), Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institut NeuroMyoGène (INMG), Université Claude Bernard Lyon 1 (UCBL), Institute of Virology, Helmholtz Zentrum Munich, German Research Center for Environmental Health, Helmholtz Zentrum München = German Research Center for Environmental Health, Biologie cellulaire des infections virales – Cell biology of viral infection, Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Helmholtz-Zentrum München (HZM), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2014
Předmět:
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Transcription
Genetic
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
05 Environmental Sciences
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Histones
Transcription (biology)
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Transcriptional regulation
Nuclear protein
Promoter Regions
Genetic
ComputingMilieux_MISCELLANEOUS
Zinc finger
Regulation of gene expression
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
0303 health sciences
030302 biochemistry & molecular biology
Nuclear Proteins
3. Good health
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
DNA-Binding Proteins
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Nucleophosmin
Biologie
Kruppel-Like Transcription Factors
Down-Regulation
Biology
DNA-binding protein
Proto-Oncogene Proteins c-myc
03 medical and health sciences
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Genetics
Humans
Protein Interaction Domains and Motifs
Transcription factor
030304 developmental biology
Cyclin-Dependent Kinase Inhibitor p15
Cell Nucleus
08 Information And Computing Sciences
Gene regulation
Chromatin and Epigenetics
Promoter
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
06 Biological Sciences
Molecular biology
Repressor Proteins
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
Alcohol Oxidoreductases
HEK293 Cells
[SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics
Epstein-Barr Virus Nuclear Antigens
Gene Expression Regulation
Hela Cells
Developmental Biology
Zdroj: Nucleic acids research
Nucleic Acids Research
Nucleic Acids Research, 2014, 42 (15), pp.9700-9716. ⟨10.1093/nar/gku697⟩
Nucleic Acids Research, Oxford University Press, 2014, 42 (15), pp.9700-9716. ⟨10.1093/nar/gku697⟩
Nucleic Acids Res. 42, 9700-9716 (2014)
ISSN: 0305-1048
1362-4962
Popis: The Epstein-Barr virus (EBV) nuclear antigen 3 family of protein is critical for the EBV-induced primary B-cell growth transformation process. Using a yeast two-hybrid screen we identified 22 novel cellular partners of the EBNA3s. Most importantly, among the newly identified partners, five are known to play direct and important roles in transcriptional regulation. Of these, the Myc-interacting zinc finger protein-1 (MIZ-1) is a transcription factor initially characterized as a binding partner of MYC. MIZ-1 activates the transcription of a number of target genes including the cell cycle inhibitor CDKN2B. Focusing on the EBNA3A/MIZ-1 interaction we demonstrate that binding occurs in EBV-infected cells expressing both proteins at endogenous physiological levels and that in the presence of EBNA3A, a significant fraction of MIZ-1 translocates from the cytoplasm to the nucleus. Moreover, we show that a trimeric complex composed of a MIZ-1 recognition DNA element, MIZ-1 and EBNA3A can be formed, and that interaction of MIZ-1 with nucleophosmin (NPM), one of its coactivator, is prevented by EBNA3A. Finally, we show that, in the presence of EBNA3A, expression of the MIZ-1 target gene, CDKN2B, is downregulated and repressive H3K27 marks are established on its promoter region suggesting that EBNA3A directly counteracts the growth inhibitory action of MIZ-1.
JOURNAL ARTICLE
SCOPUS: ar.j
info:eu-repo/semantics/published
Databáze: OpenAIRE
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