Mir-34c affects the proliferation and pluripotency of porcine induced pluripotent stem cell (piPSC)-like cells by targeting c-Myc
| Autor: | Yongxing Chang, Shuai Yu, Xiaolong Wu, Zhenshuo Zhu, Na Li, Qiaoyan Shen, Sha Peng, Juqing Zhang, Jinlian Hua, Wei Yue, Jing Sun |
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| Rok vydání: | 2021 |
| Předmět: |
Regulation of gene expression
Base Sequence Swine Cell growth Induced Pluripotent Stem Cells Transfection Biology Cell Line Cell biology Proto-Oncogene Proteins c-myc Mice MicroRNAs Gene Expression Regulation Apoptosis microRNA Animals Stem cell Induced pluripotent stem cell Cell adhesion Cell Proliferation Developmental Biology |
| Zdroj: | Cells & Development. 166:203665 |
| ISSN: | 2667-2901 |
| DOI: | 10.1016/j.cdev.2021.203665 |
| Popis: | MicroRNAs are important regulators in stem cells, which involve in gene regulation, including cell proliferation, differentiation and apoptosis. As an important one, miR-34c participates in various processes by targeting protein-coding genes. It is generally considered as a tumor suppressor and cell adhesion inhibitor. However, whether miR-34c has effects on pluripotent stem cells is not clear. Here, by mir-34c mimics transfection, the function of miR-34c on porcine induced pluripotent stem cell (piPSC)-like cells was investigated. Bioinformatics analyses showed that c-Myc is miR-34c's candidate target, which was confirmed by dual Luciferase assay. The knockout of miR-34c indicated that mir-34c affects the proliferation and pluripotency of piPSC-like cells by targeting c-Myc. Our study explored the regulatory mechanism of miR-34c on piPSC-like cells, providing a reference for the establishment of true porcine PSCs. |
| Databáze: | OpenAIRE |
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