| Autor: |
Yoshihiro Morishima, Miranda Lau, William B. Pratt, Yoichi Osawa |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
The Journal of biological chemistry. |
| ISSN: |
1083-351X |
| Popis: |
Hsp90 is known to mediate heme insertion and activation of heme-deficient neuronal NO synthase (apo-nNOS) in cells by a highly dynamic interaction that has been extremely difficult to study mechanistically with the use of subcellular systems. In that the heme content of many critical hemeproteins is regulated by Hsp90 and the heme chaperone GAPDH, the development of an in vitro system for the study of this chaperone-mediated heme regulation would be extremely useful. Here we show that use of an antibody-immobilized apo-nNOS led not only to successful assembly of chaperone complexes but the ability to show a clear dependence on Hsp90 and GAPDH for heme-mediated activation of apo-nNOS. The kinetics of binding for Hsp70 and Hsp90, the ATP- and K |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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