Symptom recovery is affected by Cucumber mosaic virus coat protein phosphorylation
| Autor: | Károly Bóka, Ákos Gellért, Pál Vági, Katalin Nemes, Katalin Salánki |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Models
Molecular Mutant Biology medicine.disease_cause Cucumovirus Virus Cucumber mosaic virus 03 medical and health sciences Downregulation and upregulation Virology Tobacco medicine Phosphorylation 030304 developmental biology Plant Diseases 0303 health sciences Mutation 030302 biochemistry & molecular biology Virion RNA Phenotype Molecular biology Plant Leaves Amino Acid Substitution Host-Pathogen Interactions Mutagenesis Site-Directed RNA Viral Capsid Proteins Protein Processing Post-Translational |
| Zdroj: | Virology. 536 |
| ISSN: | 1096-0341 |
| Popis: | Cucumber mosaic virus induces specific recovery phenotype, namely cyclic mosaic symptoms on tobacco plants. We provide further evidence that besides the 2b suppressor protein, the coat protein (CP) also has a role in symptom recovery and it is connected to its phosphorylation. We analyzed the impact of the phosphorylated (S148D) and the non-phosphorylated (S148A) state of CP148 Ser on symptom formation, virion stability and the effect of CP and its mutants on 2b-mediated local GFP-silencing. We demonstrated that a single aa change could be responsible for preventing the recovery phenomenon as replacing the phosphorylatable Ser with Ala in the 148aa position abolishing the cyclic phenomenon. CP/S148A mutation equilibrates the accumulation of the virus during the infection both at RNA and protein level in N. tabacum L. cv Xanthi plants. In summary, we determined a regulatory effect of the CMV CP on the self-attenuation mechanism and downregulation of the suppressor effect of the 2b protein. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect