Progress and Challenges in the Use of MAP1LC3 as a Legitimate Marker for Measuring Dynamic Autophagy In Vivo
| Autor: | Mario P. Tschan, Srinivasa Reddy Bonam, Sylviane Muller, Jagadeesh Bayry |
|---|---|
| Přispěvatelé: | Biotechnologie et signalisation cellulaire (BSC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche de l'Ecole de biotechnologie de Strasbourg (IREBS), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université de Paris (UP), University of Bern, Fédération Hospitalo-Universitaire (OMICARE), Centre de Recherche d’Immunologie et d’Hématologie [Strasbourg], Immuno-pathologie et immuno-intervention thérapeutique = Immunopathology and Therapeutic Immuno-intervention [CRC], Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université de Paris (UP)-École pratique des hautes études (EPHE), Institut d'Etudes Avancées de l'Université de Strasbourg, Jagadeesh, Bayry, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), Université de Strasbourg (UNISTRA)-Institut de recherche de l'Ecole de biotechnologie de Strasbourg (IREBS)-Centre National de la Recherche Scientifique (CNRS), Institut d’Etudes Avancées de l’Université de Strasbourg - Institute for Advanced Study (USIAS), Université de Strasbourg (UNISTRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
autophagy
[SDV.BIO]Life Sciences [q-bio]/Biotechnology Cell in vivo autophagy assays 610 Medicine & health Review [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology autophagic flux 03 medical and health sciences In vivo Lysosome [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology medicine Animals Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Amino Acid Sequence [SDV.BC] Life Sciences [q-bio]/Cellular Biology lcsh:QH301-705.5 030304 developmental biology 0303 health sciences Cellular process MAP1LC3 030302 biochemistry & molecular biology Autophagy Autophagosomes A protein Sciences du Vivant [q-bio]/Biotechnologies General Medicine 3. Good health Cell biology medicine.anatomical_structure lcsh:Biology (General) lysosome 570 Life sciences biology Lysosomes Microtubule-Associated Proteins Biomarkers Ex vivo Intracellular |
| Zdroj: | Cells Cells, MDPI, 2020, 9 (5), pp.1321. ⟨10.3390/cells9051321⟩ Cells, MDPI, 2020, 9, pp.1321. ⟨10.3390/cells9061321⟩ Cells, Vol 9, Iss 1321, p 1321 (2020) Cells, 2020, 9, pp.1321. ⟨10.3390/cells9061321⟩ Bonam, Srinivasa Reddy; Bayry, Jagadeesh; Tschan, Mario; Muller, Sylviane (2020). Progress and Challenges in The Use of MAP1LC3 as a Legitimate Marker for Measuring Dynamic Autophagy In Vivo. Cells, 9(5) MDPI 10.3390/cells9051321 |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells9051321⟩ |
| Popis: | International audience; Tremendous efforts have been made these last decades to increase our knowledge of intracellular degradative systems, especially in the field of autophagy. The role of autophagy in the maintenance of cell homeostasis is well documented and the existence of defects in the autophagic machinery has been largely described in diseases and aging. Determining the alterations occurring in the many forms of autophagy that coexist in cells and tissues remains complicated, as this cellular process is highly dynamic in nature and can vary from organ to organ in the same individual. Although autophagy is extensively studied, its functioning in different tissues and its links with other biological processes is still poorly understood. Several assays have been developed to monitor autophagy activity in vitro, ex vivo, and in vivo, based on different markers, the use of various inhibitors and activators, and distinct techniques. This review emphasizes the methods applied to measure (macro-)autophagy in tissue samples and in vivo via a protein, which centrally intervenes in the autophagy pathway, the microtubule-associated protein 1A/1B-light chain 3 (MAP1LC3), which is the most widely used marker and the first identified to associate with autophagosomal structures. These approaches are presented and discussed in terms of pros and cons. Some recommendations are provided to improve the reliability of the interpretation of results. |
| Databáze: | OpenAIRE |
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