Direct colorimetric method for determination of organophosphates in human urine
| Autor: | Minako Ohtani, Yoshirou Utsumi, Akira Namera, Tohru Kojima, Tohru Imamura, Mikio Yashiki |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Insecticides
Hot Temperature Pyridines Clinical Biochemistry Urine Sensitivity and Specificity Biochemistry Gas Chromatography-Mass Spectrometry Colorimetry (chemical method) chemistry.chemical_compound Organophosphorus Compounds Pyridine Acetone Humans Chelating Agents Detection limit Chromatography Biochemistry (medical) General Medicine Hydrogen-Ion Concentration Pesticide Ethylenediamines Chromogenic Compounds chemistry Spectrophotometry Reagent Colorimetry Quantitative analysis (chemistry) |
| Zdroj: | Clinica Chimica Acta. 291:9-18 |
| ISSN: | 0009-8981 |
| Popis: | A simple and sensitive method for determination of organophosphorus pesticides in human urine was developed by detecting the color complexes which resulted from reactions of organophosphorus pesticides and 4-(4-nitrobenzyl)pyridine (NBP) in urine. Based on studies of reaction conditions, e.g. reaction temperature and time, and reagent concentration, a colorimetric method was established. A 0.1-ml volume of NBP (45% in acetone) was added to a 1.0-ml volume of a urine sample, and the mixture was heated at 100 degrees C for 20 min. After cooling, 0.1 ml of tetraethylenepentamine was added. The organophosphorus pesticides showed a characteristic purplish blue color and the coloring complexes which were produced were stable for several hours. Furthermore, these complexes could be determined spectrophotometrically. The detection limits were 0.10-10 microg/ml in urine. The required time for analysis was approximately 30 min for one sample. Comparing the result of the proposed method with those of the GC-MS method, the results were similar for the 12 poisoning cases studied. Thus, the proposed method is useful for detection of these pesticides in critical care practices. |
| Databáze: | OpenAIRE |
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