Characterization of ferucarbotran-loaded RBCs as long circulating magnetic contrast agents
| Autor: | Mauro Magnani, Sonia Salamida, Carla Sfara, Antonella Antonelli, Elisabetta Manuali, Ulrich Pison, Oliver M. Weber |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Superparamagnetic iron oxide nanoparticles
Biomedical Engineering superparamagnetic iron oxides Medicine (miscellaneous) Bioengineering Spleen 02 engineering and technology Development 030218 nuclear medicine & medical imaging Long circulating 03 medical and health sciences 0302 clinical medicine Magnetic particle imaging Nuclear magnetic resonance hemic and lymphatic diseases Magnetic contrast magnetic particle spectroscopy Isotonic medicine magnetic resonance imaging General Materials Science Chemistry 021001 nanoscience & nanotechnology ferucarbotran medicine.anatomical_structure magnetic particle imaging red blood cells Tonicity 0210 nano-technology Retention time Biomedical engineering |
| Popis: | Aim: The biomedical application of contrast agents based on superparamagnetic iron oxide nanoparticles is still limited because of their short intravascular half-life. The potential of red blood cells (RBCs) loaded with new ferucarbotran nanoparticles as magnetic contrast agents with longer blood retention time has been investigated. Materials & methods: Ferucarbotran was loaded into RBCs by a procedure of hypotonic dialysis and isotonic resealing. Ferucarbotran amounts encapsulated in RBCs were determined by NMR. The survival of ferucarbotran-loaded RBCs and bulk ferucarbotran was evaluated in the mouse bloodstream. Results: Blood retention time of these RBC constructs is longer (∼14 days) than the bulk ferucarbotran (∼1 h) with a slower Fe clearance from liver and spleen. Conclusion: Ferucarbotran-loaded RBCs could be used as potential contrasting agents for diagnostic applications in MRI/magnetic particle imaging. |
| Databáze: | OpenAIRE |
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